LOCUS       sequence-87407-e        2531 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2531)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1785..1794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1791..2501
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat cgctagcgct accggactca gatctcgagc tcaagcttcg aattccatgg
       61 cggccccccg cccgtctccc gcgatccccg tttcggtctc ggctccggct ttttacgccc
      121 cgcagaagaa gttcggccct gtggtggccc caaagcccaa agtgaatccc ttccggccca
      181 gggacagcga gcctcccccg gcacccgggg cccagcgcgc acagatgggc cgggtgggcg
      241 atattccccc gccgcccccg gaagactttc ccctgcctcc acctcccctt gctggggatg
      301 gcgacgatgc agagggtgct ctgggaggtg ccttcccgcc gccccctccc ccgatcgagg
      361 aatcatttcc ccctgcgcct ctggaggagg agatcttccc ttccccgccg cctcctccgg
      421 aggaggaggg agggcctgag gcccccatac cgcccccacc acagcccagg gagaaggtga
      481 gcagtattga tttggagatc gactctctgt cctcactgct ggatgacatg accaagaatg
      541 atcctttcaa agcccgggtg tcatctggat atgtgccccc accagtggcc actccattca
      601 gttccaagtc cagtaccaag cctgcagccg ggggcacagc acccctgcct ccttggaagt
      661 ccccttccag ctcccagcct ctgccccagg ttccggctcc ggctcagagc cagacacagt
      721 tccatgttca gccccagccc cagcccaagc ctcaggtcca actccatgtc cagtcccaga
      781 cccagcctgt gtctttggct aacacccagc cccgagggcc cccagcctca tctccggctc
      841 cagcccctaa gttttctcca gtgactccta agtttactcc tgtggcttcc aagttcagtc
      901 ctggagcccc aggtggatct gggtcacaac caaatcaaaa attggggcac cccgaagctc
      961 tttctgctgg cacaggctcc cctcaacctc ccagcttcac ctatgcccag cagagggaga
     1021 agccccgagt gcaggagaag cagcaccccg tgcccccacc ggctcagaac caaaaccagg
     1081 tgcgctcccc tggggcccca gggcccctga ctctgaagga ggtggaggag ctggagcagc
     1141 tgacccagca gctaatgcag gacatggagc atcctcagag gcagaatgtg gctgtcaacg
     1201 aactctgcgg ccgatgccat caacccctgg cccgggcgca gccagccgtc cgcgctctag
     1261 ggcagctgtt ccacatcgcc tgcttcacct gccaccagtg tgcgcagcag ctccagggcc
     1321 agcagttcta cagtctggag ggggcgccgt actgcgaggg ctgttacact gacaccctgg
     1381 agaagtgtaa cacctgcggg gagcccatca ctgaccgcat gctgagggcc acgggcaagg
     1441 cctatcaccc gcactgcttc acctgtgtgg tctgcgcccg ccccctggag ggcacctcct
     1501 tcatcgtgga ccaggccaac cggccccact gtgtccccga ctaccacaag cagtacgccc
     1561 cgaggtgctc cgtctgctct gagcccatca tgcctgagcc tggccgagat gagactgtgc
     1621 gagtggtcgc cctggacaag aacttccaca tgaagtgtta caagtgtgag gactgcggga
     1681 agcccctgtc gattgaggca gatgacaatg gctgcttccc cctggacggt cacgtgctct
     1741 gtcggaagtg ccacactgct agagcccaga cggatccacc ggtcgccacc atggtgagca
     1801 agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg
     1861 agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg
     1921 acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg
     1981 acattctgac caccgcgttc agttacggca acagggcctt caccaagtac cccgacgaca
     2041 tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct
     2101 tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
     2161 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaagg
     2221 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
     2281 gcgacgtcaa gatgaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
     2341 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
     2401 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag atcgccgtgg
     2461 cccgcaactc caccgacggc atggacgagc tgtacaagta agcggccgcg actctagatc
     2521 ataatcagcc a
//
