LOCUS       sequence-87431-e        1479 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1479)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      181 cacctccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt cttacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca gctacaggac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaaca tgaacgtgtg
      481 ggacgcgtat atcacggccg acaagcagaa gaacggcatc aaagcgaact tcaagatcga
      541 gcacaacgtc gaggacggcg gcgtgcagct cgccgacgcg taccagcaga acacccccat
      601 cggcgacggc tccgtgctgc tgcctgacaa ccactacctg agcttccaga gcaagctgtt
      661 caaagacccc aacgagcagc gcgatcacat ggtcctgctg gagttcgtta ccgccgccgg
      721 gatcactccc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacatgg ctgatgactt tggcttcttc tcgtcgtcgg agagtggtgc
      841 cccggaggcg gcggaggagg acccggcggc cgccttcctg gcccagcagg agagcgagat
      901 tgcaggcata gagaacgacg agggcttcgg ggcacctgcc ggcagccatg cggcccccgc
      961 acagccgggc cccacgagtg gggctggttc tgaggacatg gggaccacag tcaatggaga
     1021 tgtgtttcag gaggccaacg gtcctgctga tggctacgca gccattgccc aggctgacag
     1081 gctgacccag gagcctgaga gcatccgcaa gtggcgagag gagcagagga aacggctgca
     1141 agagctggat gctgcatcta aggtcacgga acaggaatgg cgggagaagg ccaagaagga
     1201 cctggaggag tggaaccagc gccagagtga acaagtagag aagaacaaga tcaacaaccg
     1261 ggcatccgag gaggctttcg tgaaggaatc caaggaggag accccaggca cagagtggga
     1321 gaaggtggcc cagctatgtg acttcaaccc caagagcagc aagcagtgca aagatgtgtc
     1381 ccgcctgcgc tcggtgctca tgtccctgaa gcagacgcca ctgtcccgct agggatccac
     1441 cggatctaga taactgatca taatcagcca taccacatt
//
