LOCUS       sequence-87543-e        2225 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 530, Emission: 
            541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1468..1477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1474..2187
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tcccatgtcc
       61 accaggtccg tgtcctcgtc ctcctaccgc aggatgttcg gcggcccggg caccgcgagc
      121 cggccgagct ccagccggag ctacgtgact acgtccaccc gcacctacag cctgggcagc
      181 gcgctgcgcc ccagcaccag ccgcagcctc tacgcctcgt ccccgggcgg cgtgtatgcc
      241 acgcgctcct ctgccgtgcg cctgcggagc agcgtgcccg gggtgcggct cctgcaggac
      301 tcggtggact tctcgctggc cgacgccatc aacaccgagt tcaagaacac ccgcaccaac
      361 gagaaggtgg agctgcagga gctgaatgac cgcttcgcca actacatcga caaggtgcgc
      421 ttcctggagc agcagaataa gatcctgctg gccgagctcg agcagctcaa gggccaaggc
      481 aagtcgcgcc tgggggacct ctacgaggag gagatgcggg agctgcgccg gcaggtggac
      541 cagctaacca acgacaaagc ccgcgtcgag gtggagcgcg acaacctggc cgaggacatc
      601 atgcgcctcc gggagaaatt gcaggaggag atgcttcaga gagaggaagc cgaaaacacc
      661 ctgcaatctt tcagacagga tgttgacaat gcgtctctgg cacgtcttga ccttgaacgc
      721 aaagtggaat ctttgcaaga agagattgcc tttttgaaga aactccacga agaggaaatc
      781 caggagctgc aggctcagat tcaggaacag catgtccaaa tcgatgtgga tgtttccaag
      841 cctgacctca cggctgccct gcgtgacgta cgtcagcaat atgaaagtgt ggctgccaag
      901 aacctgcagg aggcagaaga atggtacaaa tccaagtttg ctgacctctc tgaggctgcc
      961 aaccggaaca atgacgccct gcgccaggca aagcaggagt ccactgagta ccggagacag
     1021 gtgcagtccc tcacctgtga agtggatgcc cttaaaggaa ccaatgagtc cctggaacgc
     1081 cagatgcgtg aaatggaaga gaactttgcc gttgaagctg ctaactacca agacactatt
     1141 ggccgcctgc aggatgagat tcagaatatg aaggaggaaa tggctcgtca ccttcgtgaa
     1201 taccaagacc tgctcaatgt taagatggcc cttgacattg agattgccac ctacaggaag
     1261 ctgctggaag gcgaggagag caggatttct ctgcctcttc caaacttttc ctccctgaac
     1321 ctgagggaaa ctaatctgga ttcactccct ctggttgata cccactcaaa aaggacactt
     1381 ctgattaaga cggttgaaac tagagatgga caggttatca acgaaacttc tcagcatcac
     1441 gatgaccttg aaggggatcc accggtcgcc accatggtga gcaagggcga ggggcaatcg
     1501 aagcatggac taaaggagga gatgacggtg aagtaccaca tggaaggttg cgttaatggt
     1561 cacaaatttg tcattactgg agagggcatt ggaaaccctt ttaagggtaa gcaaaccgca
     1621 aatttgtgtg tgatagaagg aggcccgctg ccgttctcgg aggacatttt aagtccgggt
     1681 tttaaatatg gtgaccggat tttcacagag tacccacagg atattgtaga ttacttcaag
     1741 aactcatgtc cagcgggcta tacgtgggaa aggagctacc tctttgagga cggagcggtc
     1801 tgtcgatgca acgtggacat aacagtctct gaaaaggaga actgcatcta tcacaaaagt
     1861 atcttcagag gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat gaccactaat
     1921 tgggaagcta gtaccgagaa aattgtgcct gttccaaagc aagggatatt aaagggaaag
     1981 gtcaaaatgt gcctgttgct gaaggatggc ggtcgttatc attgccagtt tgatacggta
     2041 tataaagcta aatcagtgcc ctccaagatg ccagaatggc attttataca gcataagctc
     2101 cttagggagg accgctctga tgctaaaaac cagaaatggc aattaacaga acatgcaatc
     2161 gcaggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     2221 cacat
//
