LOCUS       sequence-8827-ex        9898 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Ricks DM, Kutner R, Zhang XY, Welsh DA, Reiser J
  TITLE     Optimized lentiviral transduction of mouse bone marrow-derived 
            mesenchymal stem cells.
  JOURNAL   Stem Cells Dev. 2008 Jun . 17(3):441-50.
  PUBMED    18513160
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Dev. 2008 Jun . 17(3):441-50."
COMMENT     Sequence Label: pNL-EGFP/EF1alpha/WPREdU3
FEATURES             Location/Qualifiers
     source          1..9898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2429..2639
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2652..2920
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3097..3099,3100..3102,3103..3813)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3097..3099,3100..3102,3103..3813)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3142..3163)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3403..3422)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3750..3771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3833..4421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3886..3906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4304..4315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4496..4728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6370..6387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6541..7129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6621..6640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7300..8091,8092..8160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7923..7942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8161..8265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8333..8351
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8402..8424)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8510..8648
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8563..8582
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 aataaggaga gaagaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagttc tagttgaagg
     2401 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     2641 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     2701 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     2761 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     2821 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     2881 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     2941 cgtaagtgat atctactaga tttatcaaaa agagtgttga cttgtgagcg ctcacaattg
     3001 atacttagat tcatcgagag ggacacgtcg actactaacc ttcttctctt tcctacagct
     3061 gagatcaccg gtaggctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3301 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3781 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agaatcaacc
     3841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4201 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgggtacc tttaagacca
     4441 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4501 gggctaattc actcccaaag aagacaaagc tgctttttgc ctgtactggg tctctctggt
     4561 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4621 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4681 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcacc ccccaggagg
     4741 tagaggttgc agtgagccaa gatcgcgcca ctgcattcca gcctgggcaa gaaaacaaga
     4801 ctgtctaaaa taataataat aagttaaggg tattaaatat atttatacat ggaggtcata
     4861 aaaatatata tatttgggct gggcgcagtg gctcacacct gcgcccggcc ctttgggagg
     4921 ccgaggcagg tggatcacct gagtttggga gttccagacc agcctgacca acatggagaa
     4981 accccttctc tgtgtatttt tagtagattt tattttatgt gtattttatt cacaggtatt
     5041 tctggaaaac tgaaactgtt tttcctctac tctgatacca caagaatcat cagcacagag
     5101 gaagacttct gtgatcaaat gtggtgggag agggaggttt tcaccagcac atgagcagtc
     5161 agttctgccg cagactcggc gggtgtcctt cggttcagtt ccaacaccgc ctgcctggag
     5221 agaggtcaga ccacagggtg agggctcagt ccccaagaca taaacaccca agacataaac
     5281 acccaacagg tccaccccgc ctgctgccca ggcagagccg attcaccaag acgggaatta
     5341 ggatagagaa agagtaagtc acacagagcc ggctgtgcgg gagaacggag ttctattatg
     5401 actcaaatca gtctccccaa gcattcgggg atcagagttt ttaaggataa cttagtgtgt
     5461 agggggccag tgagttggag atgaaagcgt agggagtcga aggtgtcctt ttgcgccgag
     5521 tcagttcctg ggtgggggcc acaagatcgg atgagccagt ttatcaatcc gggggtgcca
     5581 gctgatccat ggagtgcagg gtctgcaaaa tatctcaagc actgattgat cttaggtttt
     5641 acaatagtga tgttacccca ggaacaattt ggggaaggtc agaatcttgt agcctgtagc
     5701 tgcatgactc ctaaaccata atttcttttt tgtttttttt tttttatttt tgagacaggg
     5761 tctcactctg tcacctaggc tggagtgcag tggtgcaatc acagctcact gcagcctcaa
     5821 cgtcgtaagc tcaagcgatc ctcccacctc agcctgcctg gtagctgaga ctacaagcga
     5881 cgccccagtt aatttttgta tttttggtag aggcagcgtt ttgccgtgtg gccctggctg
     5941 gtctcgaact cctgggctca agtgatccag cctcagcctc ccaaagtgct gggacaaccg
     6001 gggccagtca ctgcacctgg ccctaaacca taatttctaa tcttttggct aatttgttag
     6061 tcctacaaag gcagtctagt ccccaggcaa aaagggggtt tgtttcggga aagggctgtt
     6121 actgtctttg tttcaaacta taaactaagt tcctcctaaa cttagttcgg cctacaccca
     6181 ggaatgaaca aggagagctt ggaggttaga agcacgatgg aattggttag gtcagatctc
     6241 tttcactgtc tgagttataa ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc
     6301 agtcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggcgctc
     6361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6721 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6961 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8281 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8341 tatcacgagg ccctttcgtc ttcaagaact gcctcgcgcg tttcggtgat gacggtgaaa
     8401 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8461 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     8521 cccagtcacg tagcgatagc ggagtgtact ggcttaacta tgcggcatca gagcagattg
     8581 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8641 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     8701 cctcttcgct attacgccag gggaggcaga gattgcagta agctgagatc gcagcactgc
     8761 actccagcct gggcgacaga gtaagactct gtctcaaaaa taaaataaat aaatcaatca
     8821 gatattccaa tcttttcctt tatttattta tttattttct attttggaaa cacagtcctt
     8881 ccttattcca gaattacaca tatattctat ttttctttat atgctccagt tttttttaga
     8941 ccttcacctg aaatgtgtgt atacaaaatc taggccagtc cagcagagcc taaaggtaaa
     9001 aaataaaata ataaaaaata aataaaatct agctcactcc ttcacatcaa aatggagata
     9061 cagctgttag cattaaatac caaataaccc atcttgtcct caataatttt aagcgcctct
     9121 ctccaccaca tctaactcct gtcaaaggca tgtgcccctt ccgggcgctc tgctgtgctg
     9181 ccaaccaact ggcatgtgga ctctgcaggg tccctaactg ccaagcccca cagtgtgccc
     9241 tgaggctgcc ccttccttct agcggctgcc cccactcggc tttgctttcc ctagtttcag
     9301 ttacttgcgt tcagccaagg tctgaaacta ggtgcgcaca gagcggtaag actgcgagag
     9361 aaagagacca gctttacagg gggtttatca cagtgcaccc tgacagtcgt cagcctcaca
     9421 gggggtttat cacattgcac cctgacagtc gtcagcctca cagggggttt atcacagtgc
     9481 acccttacaa tcattccatt tgattcacaa tttttttagt ctctactgtg cctaacttgt
     9541 aagttaaatt tgatcagagg tgtgttccca gaggggaaaa cagtatatac agggttcagt
     9601 actatcgcat ttcaggcctc cacctgggtc ttggaatgtg tcccccgagg ggtgatgact
     9661 acctcagttg gatctccaca ggtcacagtg acacaagata accaagacac ctcccaaggc
     9721 taccacaatg ggccgccctc cacgtgcaca tggccggagg aactgccatg tcggaggtgc
     9781 aagcacacct gcgcatcaga gtccttggtg tggagggagg gaccagcgca gcttccagcc
     9841 atccacctga tgaacagaac ctagggaaag ccccagttct acttacacca ggaaaggc
//
