LOCUS       sequence-88290-e        1883 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1883)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..1883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1118..1127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1124..1126,1127..1129,1130..1840)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1169..1190)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1430..1449)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1777..1798
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga ggccaccatg gctgagcccc gccaggagtt
       61 cgaagtgatg gaagatcacg ctgggacgta cgggttgggg gacaggaaag atcagggggg
      121 ctacaccatg caccaagacc aagagggtga cacggacgct ggcctgaaag ctgaagaagc
      181 aggcattgga gacaccccca gcctggaaga cgaagctgct ggtcacgtga cccaagctcg
      241 catggtcagt aaaagcaaag acgggactgg aagcgatgac aaaaaagcca agggggctga
      301 tggtaaaacg aagatcgcca caccgcgggg agcagcccct ccaggccaga agggccaggc
      361 caacgccacc aggattccag caaaaacccc gcccgctcca aagacaccac ccagctctgg
      421 tgaacctcca aaatcagggg atcgcagcgg ctacagcagc cccggctccc caggcactcc
      481 cggcagccgc tcccgcaccc cgtcccttcc aaccccaccc acccgggagc ccaagaaggt
      541 ggcagtggtc cgtactccac ccaagtcgcc gtcttccgcc aagagccgcc tgcagacagc
      601 ccccgtgccc atgccagacc tgaagaatgt caagtccaag atcggctcca ctgagaacct
      661 gaagcaccag ccgggaggcg ggaaggtgca aatagtctac aaaccagttg acctgagcaa
      721 ggtgacctcc aagtgtggct cattaggcaa catccatcat aaaccaggag gtggccaggt
      781 ggaagtaaaa tctgagaagc ttgacttcaa ggacagagtc cagtcgaaga ttgggtccct
      841 ggacaatatc acccacgtcc ctggcggagg aaataaaaag attgaaaccc acaagctgac
      901 cttccgcgag aacgccaaag ccaagacaga ccacggggcg gagatcgtgt acaagtcgcc
      961 agtggtgtct ggggacacgt ctccacggca tctcagcaat gtctcctcca ccggcagcat
     1021 cgacatggta gactcgcccc agctcgccac gctagctgac gaggtgtctg cctccctggc
     1081 caagcagggt ttgagctccg gaggggatcc accggtcgcc accatggtga gcaagggcga
     1141 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1201 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1261 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcag
     1321 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1381 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1441 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1501 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1561 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggccaactt
     1621 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1681 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccaccagtc
     1741 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1801 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     1861 atcataatca gccataccac att
//
