LOCUS       sequence-88327-e        3255 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3255)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2499..2508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2505..3215
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2650..2668)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcggatga
       61 atttacaacc aattttctgg attggactga tcagttcagt ttgctgtgtg tttgctcaaa
      121 cagatgaaaa tagatgttta aaagcaaatg ccaaatcatg tggagaatgt atacaagcag
      181 ggccaaattg tgggtggtgc acaaattcaa catttttaca ggaaggaatg cctacttctg
      241 cacgatgtga tgatttagaa gccttaaaaa agaagggttg ccctccagat gacatagaaa
      301 atcccagagg ctccaaagat ataaagaaaa ataaaaatgt aaccaaccgt agcaaaggaa
      361 cagcagagaa gctcaagcca gaggatatta ctcagatcca accacagcag ttggttttgc
      421 gattaagatc aggggagcca cagacattta cattaaaatt caagagagct gaagactatc
      481 ccattgacct ctactacctt atggacctgt cttactcaat gaaagacgat ttggagaatg
      541 taaaaagtct tggaacagat ctgatgaatg aaatgaggag gattacttcg gacttcagaa
      601 ttggatttgg ctcatttgtg gaaaagactg tgatgcctta cattagcaca acaccagcta
      661 agctcaggaa cccttgcaca agtgaacaga actgcaccag cccatttagc tacaaaaatg
      721 tgctcagtct tactaataaa ggagaagtat ttaatgaact tgttggaaaa cagcgcatat
      781 ctggaaattt ggattctcca gaaggtggtt tcgatgccat catgcaagtt gcagtttgtg
      841 gatcactgat tggctggagg aatgttacac ggctgctggt gttttccaca gatgccgggt
      901 ttcactttgc tggagatggg aaacttggtg gcattgtttt accaaatgat ggacaatgtc
      961 acctggaaaa taatatgtac acaatgagcc attattatga ttatccttct attgctcacc
     1021 ttgtccagaa actgagtgaa aataatattc agacaatttt tgcagttact gaagaatttc
     1081 agcctgttta caaggagctg aaaaacttga tccctaagtc agcagtagga acattatctg
     1141 caaattctag caatgtaatt cagttgatca ttgatgcata caattccctt tcctcagaag
     1201 tcattttgga aaacggcaaa ttgtcagaag gagtaacaat aagttacaaa tcttactgca
     1261 agaacggggt gaatggaaca ggggaaaatg gaagaaaatg ttccaatatt tccattggag
     1321 atgaggttca atttgaaatt agcataactt caaataagtg tccaaaaaag gattctgaca
     1381 gctttaaaat taggcctctg ggctttacgg aggaagtaga ggttattctt cagtacatct
     1441 gtgaatgtga atgccaaagc gaaggcatcc ctgaaagtcc caagtgtcat gaaggaaatg
     1501 ggacatttga gtgtggcgcg tgcaggtgca atgaagggcg tgttggtaga cattgtgaat
     1561 gcagcacaga tgaagttaac agtgaagaca tggatgctta ctgcaggaaa gaaaacagtt
     1621 cagaaatctg cagtaacaat ggagagtgcg tctgcggaca gtgtgtttgt aggaagaggg
     1681 ataatacaaa tgaaatttat tctggcaaat tctgcgagtg tgataatttc aactgtgata
     1741 gatccaatgg cttaatttgt ggaggaaatg gtgtttgcaa gtgtcgtgtg tgtgagtgca
     1801 accccaacta cactggcagt gcatgtgact gttctttgga tactagtact tgtgaagcca
     1861 gcaacggaca gatctgcaat ggccggggca tctgcgagtg tggtgtctgt aagtgtacag
     1921 atccgaagtt tcaagggcaa acgtgtgaga tgtgtcagac ctgccttggt gtctgtgctg
     1981 agcataaaga atgtgttcag tgcagagcct tcaataaagg agaaaagaaa gacacatgca
     2041 cacaggaatg ttcctatttt aacattacca aggtagaaag tcgggacaaa ttaccccagc
     2101 cggtccaacc tgatcctgtg tcccattgta aggagaagga tgttgacgac tgttggttct
     2161 attttacgta ttcagtgaat gggaacaacg aggtcatggt tcatgttgtg gagaatccag
     2221 agtgtcccac tggtccagac atcattccaa ttgtagctgg tgtggttgct ggaattgttc
     2281 ttattggcct tgcattactg ctgatatgga agcttttaat gataattcat gacagaaggg
     2341 agtttgctaa atttgaaaag gagaaaatga atgccaaatg ggacacgggt gaaaatccta
     2401 tttataagag tgccgtaaca actgtggtca atccgaagta tgagggaaaa ggtagcgcat
     2461 ccgggggtgc gtcaggcggc agcggatctg gaccggtcgc caccatggtg agcaagggcg
     2521 aggaggataa catggccatc attaaggagt tcatgcgctt caaggtgcac atggaggggt
     2581 ccgtgaacgg ccacgtgttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2641 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcacc tgggacatcc
     2701 tgagccctca gttcatgtac ggctccaatg cctacgtgaa gcaccccgcc gacatccccg
     2761 actactttaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aaattcgagg
     2821 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2881 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca
     2941 tgggctggga ggccctctcc gagcggatgt accccgagga cggcgccctg aagggcgagg
     3001 tcaagcccag agtgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3061 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaaccgc aagctggaca
     3121 tcaccagcca caacgaggac tacaccatcg tggagcagta cgagagagcc gagggccgcc
     3181 actccaccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     3241 agccatacca cattt
//
