LOCUS       sequence-88409-e        1065 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-ER-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        73..124
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     75..95
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      219..228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             225..899
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 tcaatgggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
       61 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      121 agctggttta gtgaaccgtc agatccgcta gcgccaccat gctgctatcc gtgccgttgc
      181 tgctcggcct cctcggcctg gccgtcgccg caccggtcgc caccatggtg agtgtgatta
      241 aaccagacat gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga
      301 ttgaaggagt tggccttggg aagcctttcg agggaaaaca gagtatggac cttaaagtca
      361 aagaaggcgg acctctgcct ttcgcctatg acatcttgac aactgtgttc tgttacggca
      421 acagggtatt cgccaaatac ccagaaaata tagtagacta tttcaagcag tcgtttcctg
      481 agggctactc ttgggaacga agcatgaatt acgaagacgg gggcatttgt aacgcgacaa
      541 acgacataac cctggatggt gactgttata tctatgaaat tcgatttgat ggtgtgaact
      601 ttcctgccaa tggtccagtt atgcagaaga ggactgtgaa atgggagcca tccactgaga
      661 aattgtatgt gcgtgatgga gtgctgaagg gtgatgttaa catggctctg tcgcttgaag
      721 gaggtggcca ttaccgatgt gacttcaaaa ctacttataa agctaagaag gttgtccagt
      781 tgccagacta tcactttgtg gaccaccaca ttgagattaa aagccacgac aaagattaca
      841 gtaatgttaa tctgcatgag cacgccgaag cgcattctga gctgccgagg caggccaagt
      901 ccggaaagga cgagctgtaa gaattctgca gtcgacggta ccgcgggccc gggatccacc
      961 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1021 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaa
//
