LOCUS       sequence-88437-e        7163 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7163)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagacatg
       61 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
      121 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
      181 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
      241 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
      301 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
      361 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      421 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
      481 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
      541 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
      601 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
      661 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagtc cggactcaga
      721 tctcgagctc aagctagctt cgaattcgcc accatgcagc agtacaaccg agtggggaaa
      781 gtggagcacg gctctgtggc tctgccagcc atcatgcgct ctggagcctc tggtcctgag
      841 aatttccagg tggggagcat gccacctgcc cagcagcaga tcaccagtgg ccagatgcac
      901 cgaggacaca tgccacctct gacttcagcc cagcaggcgc tcactggaac cattaactcc
      961 agcatgcagg ctgtgcaggc tgcccaggcc actctggatg actttgagac tctaccccct
     1021 cttggccagg atgctgcctc caaagcttgg cgtaagaaca agatggatga atcaaagcat
     1081 gagatccact cccaggtaga tgccatcaca gctggcactg cctctgtggt aaacctgaca
     1141 gcaggagatc ctgcagagac agactataca gcagtgggct gtgcagtcac caccatctct
     1201 tccaacctga cggagatgtc ccgtggggtg aagctattgg ctgccctgct ggaggatgaa
     1261 ggcggcaatg gccggcccct tctgcaagca gcaaagggcc ttgcaggggc tgtgtcggaa
     1321 ctgcttcgca gtgctcagcc cgccagcgct gagcctcgtc agaacctgct gcaggcagcc
     1381 gggaacgtgg gccaggccag tggggagctg ttgcagcaaa ttggggaaag tgacactgac
     1441 ccccacttcc aggatgttct aatgcagcta gcaaacgcag tggcaagtgc tgcagccgcc
     1501 ctggtcctca aggccaagag tgtggcccag cgcacagagg attccgggct tcagacccaa
     1561 gtgattgctg cagctacgca atgtgctctg tccacttccc agctggtggc ctgtaccaag
     1621 gtggtagcac ctacaatcag ctcacctgtg tgccaagaac agctagtcga ggctgggcga
     1681 ctggtggcca aagctgtgga gggctgtgtg tctgcctcgc aagcagctac agaagatgga
     1741 cagctgcttc gaggggtagg agcagcagcc acggctgtca cccaggccct caatgagctg
     1801 ctgcagcacg tgaaggccca cgccaccgga gctgggcctg ccggtcggta tgaccaagcc
     1861 actgatacca tcctcaccgt cactgaaaac atcttcagct ccatgggtga tgctggggag
     1921 atggtgcgcc aggcccgcat cctggcccaa gccacctcag acctggtcaa tgctatcaag
     1981 gccgatgctg agggggagag tgatctggag aactctagaa agctcctgag tgctgccaag
     2041 atcctcgctg atgccaccgc caagatggtg gaggcggcca agggagcagc cgcccaccct
     2101 gacagtgagg aacagcagca gcgactgcgt gaagcagctg aggggcttcg catggccacc
     2161 aatgcagctg cgcagaacgc catcaagaag aagttggtgc agcgcctgga gcatgcagcc
     2221 aagcaagctg cagcctctgc aacacagacc attgctgcag cccaacatgc agcctctgcc
     2281 cccaaggcct ctgcgggccc ccagccccta ctggtgcaga gctgtaaggc cgtggcagag
     2341 cagattccgt tgctggtgca gggtgtccga ggaagtcaag ctcagcctga cagccctagt
     2401 gctcagctcg ccctcattgc tgccagccag agcttcctgc agccaggtgg gaagatggtg
     2461 gcagcggcaa aagcctcagt gccaacaatt caggaccagg cttcagcgat gcagctgagt
     2521 cagtgcgcaa agaacctagg cactgccctg gccgagctgc gcactgctgc tcagaaggct
     2581 caggaggcat gtggaccttt ggagatggat tctgcactga gtgttgtaca aaatctagag
     2641 aaagacctac aggaaataaa agcagcagct cgagatggca agcttaagcc cttacctggg
     2701 gaaacaatgg agaagtgtac ccaagatctt ggcaacagca ccaaagcggt gagctctgcc
     2761 atcgccaagc tgctggggga gattgcccag ggcaatgaga actatgcagg tattgcagct
     2821 cgggatgtag ctggtggact aaggtcacta gcccaggctg cgcggggtgt ggctgcgctg
     2881 acatcagatc ctgcagtgca ggccattgtg cttgacacag ccagcgacgt tctggacaag
     2941 gccagcagcc tcattgaaga ggcaaaaaaa gcatctggac acccagggga cccagaaagc
     3001 cagcaaaggc ttgctcaggt agctaaagca gtgacccaag ccctgaaccg ctgtgtcagc
     3061 tgcctgcctg gccaaagaga cgtggataat gccctacgag ccgttggaga tgccagcaag
     3121 cgactcctga gtgacttgct tcctccaagc acggggacat ttcaagaagc acagagccga
     3181 ttgaatgaag ctgcagctgg gttaaatcag gccgccacag aactggtgca agcctcccga
     3241 ggaactcctc aggacctggc tcgggcctcc ggccgatttg gacaggactt cagcaccttc
     3301 ctggaagctg gtgtggagat ggccggacag gccccgagcc aggaggaccg agcccaggtg
     3361 gtgtccaacc tgaagggcat atccatgtct tcaagcaaac ttctccttgc cgctaaggcc
     3421 ttgtccacag accctgcttc tcccaacctc aagagtcagc tggctgcggc tgcccgggca
     3481 gtgacggaca gcatcaacca gctcatcacc atgtgcaccc agcaggcacc tggccagaag
     3541 gagtgtgaca atgcacttcg gcagctagag acagtccgag aactcctgga gaatccagtc
     3601 cagcccatca acgacatgtc ctacttcggt tgcttggaca gtgtcatgga gaactccaag
     3661 gtcctaggtg aggccatgac tggcatctcc caaaatgcca agaatggaaa tctgccggag
     3721 tttggagatg ccattgccac agcctccaag gctctctgtg gcttcacgga ggcagccgca
     3781 caggcagcat atctagttgg tgtctctgac cccaacagcc aagctggaca gcaaggactg
     3841 gtggaaccca cacagtttgc ccgtgcaaac caggcaattc agatggcctg tcagagtctg
     3901 ggggagcctg gctgtaccca ggcccaggtg ttatctgcag ccactattgt agccaaacac
     3961 acatctgcat tgtgtaacag ctgtcgcctg gcttccgcta ggactgccaa tcccactgcc
     4021 aagcgccagt ttgtacagtc agccaaggag gtggccaaca gtacagccaa tcttgtcaag
     4081 accatcaagg cactagatgg ggacttcaca gaagagaacc gtgcccagtg ccgagcagcc
     4141 acagcccctc tgctggaagc tgtggataac ctgagtgcct ttgcctccaa ccctgagttc
     4201 tccagcgtcc ctgcccagat cagccctgag ggcagagcag ccatggagcc cattgtaatc
     4261 tctgctaaga caatgttgga gagtgctgga gggctcatcc agacagctcg ggcgttagca
     4321 gtcaatcccc gagacccccc acgttggtct gtgctagctg gccactctcg gactgtctca
     4381 gattccatca agaaacttat tacaagcatg agggacaaag ccccagggca gctggagtgt
     4441 gagacagcca ttgcggctct gaacagctgc ttgcgggacc tagatcaggc ttcgcttgcc
     4501 gctgtcagcc agcagcttgc tccccgtgaa ggaatctctc aagaggcttt gcacacccag
     4561 atgctcactg cagtgcaaga aatttctcat ctcattgagc cgctggccag cgctgctcgg
     4621 gctgaagcct cccagctggg acacaaggtg tcccaaatgg cccagtactt tgaaccactc
     4681 accctggctg cagtgggtgc tgcgtctaag accctgagcc acccgcagca gatggcactt
     4741 ctggaccaga ctaaaacgtt ggcagagtct gccttgcagt tgctatatac tgccaaggag
     4801 gctggtggta accccaagca agcagcacac acccaggaag ccctggagga ggctgtgcag
     4861 atgatgacag aggccgtaga agacctgacg acgaccctca acgaagcagc cagtgctgca
     4921 ggagtcgttg gcggcatggt ggactctatc actcaggcca tcaaccagct agatgaagga
     4981 cctatgggtg acccagaagg ctcattcgta gattaccaga caaccatggt gaggacagcc
     5041 aaggccattg ctgtcactgt tcaggagatg gtaaccaagt caaacaccag ccctgaagag
     5101 ctaggccctc ttgccaacca gctgaccagt gactatggcc gactggcctc acaagccaag
     5161 cctgcagctg tggctgctga aaatgaagag ataggcgctc acatcaaaca ccgagtacag
     5221 gagctgggcc atggctgctc tgctctggtc accaaggcag gtgccttgca gtgtagcccc
     5281 agtgatgtct acaccaagaa ggagctcata gagtgtgccc gcagagtgtc agagaaggtc
     5341 tcccatgttc tggctgcact ccaggctggg aatcgtggta cccaggcctg cattacagca
     5401 gccagtgctg tgtctggtat cattgctgac ctcgacacca ccatcatgtt cgctactgct
     5461 ggcacactta accgtgaggg tgctgaaact tttgctgacc accgggaggg tatcttaaag
     5521 acagcaaagg ttcttgtgga ggacaccaag gtcctagtgc agaatgcagc tgggagccag
     5581 gagaagttgg cacaagccgc ccagtcctcc gtggccacca ttacccgcct cgctgatgtg
     5641 gtcaagctcg gtgcagccag cctaggagcc gaagaccctg aaactcaggt ggtgctgatc
     5701 aatgcagtaa aggacgtagc caaggccctg ggtgacctca tcagcgctac gaaggctgca
     5761 gcgggcaaag ttggggatga ccctgcagtg tggcagctca agaactctgc caaggtgatg
     5821 gtgaccaatg tgacatcatt gctcaagaca gtgaaggctg tggaagatga ggccaccaaa
     5881 ggcacacggg ccctagagaa gacctctacc cctgaagact tcatccgaat gaccaagggt
     5941 attactatgg caacagccaa agccgttgct gctggcaatt cctgtcgaca ggaagatgtc
     6001 attgccacag ccaatctgag ccgacgggct attgcggaca tgcttcgggc ttgcaaggaa
     6061 gcagctttcc acccagaagt ggcgcctgat gtacggctcc gagccctgca ctatggccgg
     6121 gagtgtgcca atggttacct ggaactgctg gaccacgtgc tgctgaccct tcagaagcca
     6181 aacccagacc tgaagcagca gctaactgga cactcaaagc gggttgctgg ctctgtgact
     6241 gagctcatcc aggctgctga agccatgaaa ggaacagagt gggtggaccc agaggaccct
     6301 actgtcattg ctgagaatga gctcttggga gccgcagccg ccatcgaggc tgcagccaag
     6361 aagctggagc agctgaagcc cagggccaaa cccaaggagg cggatgagtc cttgaacttt
     6421 gaggaacaaa tcctagaagc tgccaagtcc atcgctgcag ccaccagtgc actggtaaag
     6481 gctgcgtcag cagcccagag ggagctggtg gctcaaggaa aggtgggcgc cattccagcc
     6541 aatgcactgg atgatgggca gtggtcgcag ggcctcattt ccgctgcccg tatggtggct
     6601 gcggccacca acaatctgtg tgaagcagct aacgcagctg tccagggcca tgctagccag
     6661 gagaaactca tttcctcagc caagcaagta gctgcctcca cagctcagct cctggtagct
     6721 tgcaaggtca aagcagatca ggactctgag gcaatgaaac ggctccaggc tgctggcaat
     6781 gcagtgaaga gggcctcaga taacctggta aaggcggccc agaaagctgc agccttcgaa
     6841 gaccaggaga atgagacggt ggtggtgaag gagaaaatgg ttgggggcat tgcccagatt
     6901 atcgcagcac aggaagagat gcttcggaag gaacgagagc tggaagaggc tcggaaaaag
     6961 ctcgcccaga tccggcagca gcagtacaag ttcttgcctt cagagcttcg agacgagcac
     7021 taaaaaagcc ctgtgtattt aatgcagacc cagcccagag actgtgcctg ccactaccaa
     7081 agccttctgg gctgctcagg acccaagctg cccaacccca gcactccccc aaagtgcctg
     7141 ccaaaccctg ggcctggccc tgc
//
