LOCUS       sequence-88657-e        2188 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2188)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1457..1466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1463..2146
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gacggtcaga tccgctagcg atgccaccat ggccaaaaga aatgccgaga aggaactgac
       61 agataggaat tgggatcaag aagatgaagc tgaagaggtg ggaacattct ccatggccag
      121 tgaggaagtc ttgaagaata gagccataaa gaaagcaaag cgcagaaatg ttggatttga
      181 atctgacact ggaggagcct ttaaaggttt taaaggtttg gtggtacctt ctggaggagg
      241 acgcttttct ggatttggta gtggcgctgg agggaagcct ctggaaggac tgtcgaatgg
      301 aaacaacata accagtgccc ctcccttcgc cagtgcaaag gcagcggcag atcccaaggt
      361 agcctttggt tctcttgctg caaatggccc taccaccttg gttgataaag tttcaaatcc
      421 caaaactaat ggggacagtc agcagccctc ctcctctggc cttgcttcca gtaaagcttg
      481 tgtcggaaat gcctatcaca agcagttggc cgccttgaac tgctccgtgc gggattggat
      541 agtgaagcac gtgaatacaa accccctctg tgatctgaca cctatcttta aagactatga
      601 gaaatattta gcaaacattg aacagcaaca cgggaacagt ggcaggaatt ctgaaagtga
      661 atctaacaaa gtggcagctg aaacacagtc tccttccctt tttggctcaa caaaattaca
      721 gcaagagtca acgtttttgt ttcatggcaa caaaactgaa gatacacctg acaagaagat
      781 ggaggtggca tctgaaaaga aaacggaccc atcatcacta ggagcgacaa gtgcctcatt
      841 taatttcggc aagaaagttg atagctctgt tttgggctca ttaagctctg tccccctgac
      901 tggattttct ttctcccctg gaaactccag tttatttggc aaagatacta cccagagtaa
      961 accagtctct tcaccatttc ccactaaacc attggagggc caagcagaag gtgacagtgg
     1021 tgaatgcaaa ggtggagatg aagaagagaa tgatgagcca cccaaagtag tagttaccga
     1081 agtaaaagaa gaagacgctt tttactccaa aaagtgtaaa ctgttttaca agaaagacaa
     1141 tgagtttaaa gagaaaggca taggtactct gcatttaaaa cctacagcaa atcagaagac
     1201 acagcttttg gtgcgggcag acaccaattt aggcaacata ttgctgaacg ttctgattcc
     1261 acccaatatg ccatgtacgc gaacagggaa gaataacgtt cttatcgtct gtgttccaaa
     1321 tccaccaatt gacgagaaga atgccaccat gccagtcacc atgttgattc gggtaaaaac
     1381 cagcgaggat gcagacgagt tgcacaaaat tttactggag aaaaaggatg ccagctccgg
     1441 aggggatcca ccggtcgcca ccatggtgag tgcgattaag ccagacatga ggatcaaact
     1501 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
     1561 gccttatgag ggaaaacaga ccatggatct tgaagtcaaa gagggcggac ctctgccttt
     1621 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg tgaaatatcc
     1681 agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
     1741 cttgactttc gaagacgggg gcatttgcaa tgccagaaac gacataacaa tggaagggga
     1801 cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat
     1861 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
     1921 gctgacgggt gatattgaga tggctttgtt gcttgaagga aatgcccatt accgatgtga
     1981 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg cccactttgt
     2041 ggaccacgcc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
     2101 gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct
     2161 agatcataat cagccatacc acattgtg
//
