LOCUS       sequence-88686-e        2074 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 503 / 569, 
            Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2074)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-Calreticulin-N-16
FEATURES             Location/Qualifiers
     source          1..2074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1349..2029
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 tgcccgtcag atccgctagc gctaccggac tcaagatctc gaggccacca tgctgctatc
       61 cgtgccgctg ctgctcggcc tcctcggcct ggccgtcgcc gagcctgccg tctacttcaa
      121 ggagcagttt ctggacggag acgggtggac ttcccgctgg atcgaatcca aacacaagtc
      181 agattttggc aaattcgttc tcagttccgg caagttctac ggtgacgagg agaaagataa
      241 aggtttgcag acaagccagg atgcacgctt ttatgctctg tcggccagtt tcgagccttt
      301 cagcaacaaa ggccagacgc tggtggtgca gttcacggtg aaacatgagc agaacatcga
      361 ctgtgggggc ggctatgtga agctgtttcc taatagtttg gaccagacag acatgcacgg
      421 agactcagaa tacaacatca tgtttggtcc cgacatctgt ggccctggca ccaagaaggt
      481 tcatgtcatc ttcaactaca agggcaagaa cgtgctgatc aacaaggaca tccgttgcaa
      541 ggatgatgag tttacacacc tgtacacact gattgtgcgg ccagacaaca cctatgaggt
      601 gaagattgac aacagccagg tggagtccgg ctccttggaa gacgattggg acttcctgcc
      661 acccaagaag ataaaggatc ctgatgcttc aaaaccggaa gactgggatg agcgggccaa
      721 gatcgatgat cccacagact ccaagcctga ggactgggac aagcccgagc atatccctga
      781 ccctgatgct aagaagcccg aggactggga tgaagagatg gacggagagt gggaaccccc
      841 agtgattcag aaccctgagt acaagggtga gtggaagccc cggcagatcg acaacccaga
      901 ttacaagggc acttggatcc acccagaaat tgacaacccc gagtattctc ccgatcccag
      961 tatctatgcc tatgataact ttggcgtgct gggcctggac ctctggcagg tcaagtctgg
     1021 caccatcttt gacaacttcc tcatcaccaa cgatgaggca tacgctgagg agtttggcaa
     1081 cgagacgtgg ggcgtaacaa aggcagcaga gaaacaaatg aaggacaaac aggacgagga
     1141 gcagaggctt aaggaggagg aagaagacaa gaaacgcaaa gaggaggagg aggcagagga
     1201 caaggaggat gatgaggaca aagatgagga tgaggaggat gaggaggaca aggaggaaga
     1261 tgaggaggaa gatgtccccg gccaggccaa ggacgagctg gcatccgggg gtgcgtcagg
     1321 cggcagcgga tctggaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa
     1381 actccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
     1441 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
     1501 ttttgccttt gatatcctga ccactgcatt cctgtacggc aacagggtat tcgccaaata
     1561 tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
     1621 aagcttgact ttcgaagacg ggggcatttg caacgccaga aacgacataa caatggaagg
     1681 ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt
     1741 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
     1801 agtgctgacg ggtgatgtag agatggcttt gttgcttgaa ggaaatgccc attaccgatg
     1861 tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag gcgcccactt
     1921 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
     1981 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac
     2041 tctagatcat aatcagccat accacattgt agag
//
