LOCUS       sequence-88705-e        1230 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Mitochondrial Membrane, Excitation: 503 / 569, 
            Emission: 514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             501..1181
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 ccgatgctag tgacgtcaga tccgctagcg ccaccatggt gggtcggaac agcgccatcg
       61 ccgccggtgt atgcggggcc cttttcattg ggtactgcat ctacttcgac cgcaaaagac
      121 gaagtgaccc caacttcaag aacaggcttc gagaacgaag aaagaaacag aagcttgcca
      181 aggagagagc tgggctttcc aagttacctg accttaaaga tgctgaagct gttcagaagt
      241 tcttccttga agaaatacag cttggtgaag agttactagc tcaaggtgaa tatgagaagg
      301 gcgtagacca tctgacaaat gcaattgctg tgtgtggaca gccacagcag ttactgcagg
      361 tcttacagca aactcttcca ccaccagtgt tccagatgct tctgactaag ctcccaacaa
      421 ttagtcagag aattgtaagt gctcagagct tggctgaaga tgatgtggaa ggcggtagcg
      481 gggatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc aaactccgta
      541 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
      601 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
      661 ttgatatcct gaccactgca ttcctgtacg gcaacagggt attcgccaaa tatccagaca
      721 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
      781 ctttcgaaga cgggggcatt tgcaacgcca gaaacgacat aacaatggaa ggggacactt
      841 tctataataa agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga
      901 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
      961 cgggtgatgt agagatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
     1021 gaactactta caaagctaag gagaagggtg tcaagttacc aggcgcccac tttgtggacc
     1081 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
     1141 ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc
     1201 ataatcagcc ataccacatt gagagaatcc
//
