LOCUS       sequence-88769-e        2945 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2945)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..2945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1503..2180
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2229..2906
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 ggacggtcag atccgctagc gccaccatgg gctgtgtgca atgtaaggat aaagaagcaa
       61 caaaactgac ggaggagagg gacggcagcc tgaaccagag ctctgggtac cgctatggca
      121 cagaccccac ccctcagcac taccccagct tcggtgtgac ctccatcccc aactacaaca
      181 acttccacgc agccgggggc caaggactca ccgtctttgg aggtgtgaac tcttcgtctc
      241 atacggggac cttgcgtacg agaggaggaa caggagtgac actctttgtg gccctttatg
      301 actatgaagc acggacagaa gatgacctga gttttcacaa aggagaaaaa tttcaaatat
      361 tgaacagctc ggaaggagat tggtgggaag cccgctcctt gacaactgga gagacaggtt
      421 acattcccag caattatgtg gctccagttg actctatcca ggcagaagag tggtactttg
      481 gaaaacttgg ccgaaaagat gctgagcgac agctattgtc ctttggaaac ccaagaggta
      541 cctttcttat ccgcgagagt gaaaccacca aaggtgccta ttcactttct atccgtgatt
      601 gggatgatat gaaaggagac catgtcaaac attataaaat tcgcaaactt gacaatggtg
      661 gatactacat taccacccgg gcccagtttg aaacacttca gcagcttgta caacattact
      721 caggtacctg gaatggaaac acaaaagtag ccataaagac tcttaaacca ggcacaatgt
      781 cccccgaatc attccttgag gaagcgcaga tcatgaagaa gctgaagcac gacaagctgg
      841 tccagctcta tgcagtggtg tctgaggagc ccatctacat cgtcaccgag tatatgaaca
      901 aaggaagttt actggatttc ttaaaagatg gagaaggaag agctctgaaa ttaccaaatc
      961 ttgtggacat ggcagcacag gtggctgcag gaatggctta catcgagcgc atgaattata
     1021 tccatagaga tctgcgatca gcaaacattc tagtggggaa tggactcata tgcaagattg
     1081 ctgacttcgg attggcccga ttgatagaag acaatgagta cacagcaaga caaggtgcaa
     1141 agttccccat caagtggacg gcccccgagg cagccctgta cgggaggttc acaatcaagt
     1201 ctgacgtgtg gtcttttgga atcttactca cagagctggt caccaaagga agagtgccat
     1261 acccaggcat gaacaaccgg gaggtgctgg agcaggtgga gcgaggctac aggatgccct
     1321 gcccgcagga ctgccccatc tctctgcatg agctcatgat ccactgctgg aaaaaggacc
     1381 ctgaagaacg ccccactttt gagtacttgc agagcttcct ggaagactac tttaccgcga
     1441 cagagcccca gtaccaacct ggtgaaaacc tggcatccag cggggatcca ccggtcgcca
     1501 ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc aacgtaaacg
     1561 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta
     1621 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
     1681 cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa gactatttta
     1741 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
     1801 tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
     1861 ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
     1921 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat attcgcatgg
     1981 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
     2041 aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt gagattttaa
     2101 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
     2161 tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact ggttctggtt
     2221 cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa ggcaacgtaa
     2281 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
     2341 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
     2401 ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata caagactatt
     2461 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
     2521 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
     2581 gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
     2641 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt gatattcgca
     2701 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
     2761 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
     2821 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
     2881 gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc
     2941 acatt
//
