LOCUS       sequence-88799-e        7454 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7454)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-MyosinIIB-C-18
FEATURES             Location/Qualifiers
     source          1..7454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..706
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             755..1429
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa
       61 cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
      121 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
      181 ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata
      241 tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
      301 aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg
      361 ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt
      421 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
      481 agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg
      541 tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt
      601 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
      661 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg gtagcggtca
      721 cggtactggt tctactggtt ctggttcttc tgagagtgcg attaagccag acatgaagat
      781 caacctccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
      841 aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct
      901 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccga
      961 atatccagac cacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
     1021 acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga
     1081 aggggacact ttctataata aagttcgatt tcacggtgta aactttcccg ccaatggtcc
     1141 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
     1201 tggagtgctg actggtgata ttcgcatggc tttgttgctt gaaggaaatg cccattaccg
     1261 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca
     1321 ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct
     1381 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat ccggaggtag
     1441 cgcatccagt gggggtgcgt caggcagtgg cagcggatct ggcgcgcaga gaactggact
     1501 cgaggatcca gagaggtatc tctttgtgga cagggctgtc atctacaacc ctgccactca
     1561 agctgattgg acagctaaaa agctagtgtg gattccatca gaacgccatg gttttgaggc
     1621 agctagtatc aaagaagaac ggggagatga agttatggtg gagttggcag agaatggaaa
     1681 gaaagcaatg gtcaacaaag atgatattca gaagatgaac ccacctaagt tttccaaggt
     1741 ggaggatatg gcagaattga catgcttgaa tgaagcttcc gttttacata atctgaagga
     1801 tcgctactat tcaggactaa tctatactta ttctggactc ttctgtgtag ttataaaccc
     1861 ttacaagaat cttccaattt actctgagaa tattattgaa atgtacagag ggaagaagcg
     1921 tcatgagatg cctccacaca tctatgctat atctgaatct gcttacagat gcatgcttca
     1981 agatcgtgag gaccagtcaa ttctttgcac gggtgagtca ggtgctggga agacagaaaa
     2041 tacaaagaaa gttattcagt accttgccca tgttgcttct tcacataaag gaagaaagga
     2101 ccataatatt cctggggaac ttgaacggca gcttttgcaa gcaaatccaa ttctggaatc
     2161 atttggaaat gcgaagactg tgaaaaatga taactcatct cgttttggca aatttattcg
     2221 gatcaacttt gatgtaactg gctatatcgt tggggccaac attgaaacat accttctgga
     2281 aaagtctcgt gctgttcgtc aagcaaaaga tgaacgtact tttcatatct tttaccagtt
     2341 gttatctgga gcaggagaac acctaaagtc tgatttgctt cttgaaggat ttaataacta
     2401 caggtttctc tccaatggct atattcctat tccgggacag caagacaaag ataatttcca
     2461 ggagaccatg gaagcaatgc acataatggg cttctcccat gaagagattc tgtcaatgct
     2521 taaagtagta tcttcagtgc tacagtttgg aaatatttct ttcaaaaagg agagaaatac
     2581 tgatcaagct tccatgccag aaaatacagt tgcgcagaag ctctgccatc ttcttgggat
     2641 gaatgtgatg gagtttactc gggccatcct gactccccgg atcaaggtcg gccgagacta
     2701 tgtgcaaaaa gcccagacca aagaacaggc agattttgca gtagaagcat tggcaaaagc
     2761 tacctatgag cggctctttc gctggctcgt tcatcgcatc aataaagctc tggataggac
     2821 caaacgtcag ggagcatctt tcattggaat cctggatatt gctggatttg aaatttttga
     2881 gctgaactcc tttgaacaac tttgcatcaa ctacaccaat gagaagctgc agcagctgtt
     2941 caaccacacc atgtttatcc tagaacaaga ggaataccag cgcgaaggca tcgagtggaa
     3001 cttcatcgat ttcgggctgg atctgcagcc atgcatcgac ctaatagaga gacctgcgaa
     3061 ccctcctggt gtactggccc ttttggatga agaatgctgg ttccctaaag ccacagataa
     3121 aacctttgtt gaaaaactgg ttcaagagca aggttcccac tccaagtttc agaaacctcg
     3181 acaattaaaa gacaaagctg atttttgcat tatacattat gcagggaagg tggactataa
     3241 ggcagatgag tggctgatga agaatatgga ccccctgaat gacaacgtgg ccaccctttt
     3301 gcaccagtca tcagacagat ttgtggcaga gctttggaaa gatgtggacc gtatcgtggg
     3361 tctggatcaa gtcactggta tgactgagac agcttttggc tccgcatata aaaccaagaa
     3421 gggcatgttt cgtaccgttg ggcaactcta caaagaatct ctcaccaagc tgatggcaac
     3481 tctccgaaac accaacccta actttgttcg ttgtatcatt ccaaatcacg agaagagggc
     3541 tggaaaattg gatccacacc tagtcctaga tcagcttcgc tgtaatggtg tcctggaagg
     3601 gatccgaatc tgtcgccagg gcttccctaa ccgaatagtt ttccaggaat tcagacagag
     3661 atatgagatc ctaactccaa atgctattcc taaaggtttt atggatggta aacaggcctg
     3721 tgaacgaatg atccgggctt tagaattgga cccaaacttg tacagaattg gacagagcaa
     3781 gatatttttc agagctggag ttctggcaca cttagaggaa gaaagagatt taaaaatcac
     3841 cgatatcatt atcttcttcc aggccgtttg cagaggttac ctggccagaa aggcctttgc
     3901 caagaagcag cagcaactaa gtgccttaaa ggtcttgcag cggaactgtg ccgcgtacct
     3961 gaaattacgg cactggcagt ggtggcgagt cttcacaaag gtgaagccgc ttctacaagt
     4021 gactcgccag gaggaagaac ttcaggccaa agatgaagag ctgttgaagg tgaaggagaa
     4081 gcagacaaag gtggaaggag agctggagga gatggagcgg aagcaccagc agcttttaga
     4141 agagaagaat atccttgcag aacaactaca agcagagact gagctctttg ctgaagcaga
     4201 agagatgagg gcaagacttg ctgctaaaaa gcaggaatta gaagagattc tacatgactt
     4261 ggagtctagg gttgaagaag aagaagaaag aaaccaaatc ctccaaaatg aaaagaaaaa
     4321 aatgcaagca catattcagg acctggaaga acagctagac gaggaggaag gggctcggca
     4381 aaagctgcag ctggaaaagg tgacagcaga ggccaagatc aagaagatgg aagaggagat
     4441 tctgcttctc gaggaccaaa attccaagtt catcaaagaa aagaaactca tggaagatcg
     4501 cattgctgag tgttcctctc agctggctga agaggaagaa aaggcgaaaa acttggccaa
     4561 aatcaggaat aagcaagaag tgatgatctc agatctagaa gaacgcttaa agaaagaaga
     4621 aaagactcgt caggaactgg aaaaggccaa aagaaaactc gacggggaga cgaccgacct
     4681 gcaggaccag atcgcagagc tgcaggcgca gattgatgag ctcaagctgc agctggccaa
     4741 gaaggaggag gagctgcagg gcgcactggc cagaggtgat gatgaaacac tccataagaa
     4801 caatgccctt aaagttgtgc gagagctaca agcccaaatt gctgaacttc aggaagactt
     4861 tgaatccgag aaggcttcac ggaacaaggc cgaaaagcag aaaagggact tgagtgagga
     4921 actggaagct ctgaaaacag agctggagga cacgctggac accacggcag cccagcagga
     4981 actacgtaca aaacgtgaac aagaagtggc agagctgaag aaagctcttg aggaggaaac
     5041 taagaaccat gaagctcaaa tccaggacat gagacaaaga cacgcaacag ccctggagga
     5101 gctctcagag cagctggaac aggccaagcg gttcaaagca aatctagaga agaacaagca
     5161 gggcctggag acagataaca aggagctggc gtgtgaggtg aaggtcctgc agcaggtcaa
     5221 ggctgagtct gagcacaaga ggaagaagct cgacgcgcag gtccaggagc tccatgccaa
     5281 ggtctctgaa ggcgacaggc tcagggtgga gctggcggag aaagcaagta agctgcagaa
     5341 tgagctagat aatgtctcca cccttctgga agaagcagag aagaagggta ttaaatttgc
     5401 taaggatgca gctagtcttg agtctcaact acaggataca caggagcttc ttcaggagga
     5461 gacacgccag aaactaaacc tgagcagtcg gatccggcag ctggaagagg agaagaacag
     5521 tcttcaggag cagcaggagg aggaggagga ggccaggaag aacctggaga agcaagtgct
     5581 ggccctgcag tcccagttgg ctgataccaa gaagaaagta gatgacgacc tgggaacaat
     5641 tgaaagtctg gaagaagcca agaagaagct tctgaaggac gcggaggccc tgagccagcg
     5701 cctggaggag aaggcactgg cgtatgacaa actggagaag accaagaacc gcctgcagca
     5761 ggagctggac gacctcacgg tggacctgga ccaccagcgc caggtcgcct ccaacttgga
     5821 gaagaagcag aagaagtttg accagctgtt agcagaagag aagagcatct ctgctcgcta
     5881 tgccgaagag cgggaccggg ccgaagccga ggccagagag aaagaaacca aagccctgtc
     5941 actggcccgg gccctcgagg aagccctgga ggccaaggag gagtttgaga ggcagaacaa
     6001 gcagctccga gcagacatgg aagacctcat gagctccaaa gatgatgtgg gaaaaaacgt
     6061 tcacgaactt gaaaaatcca aacgggccct agagcagcag gtggaggaaa tgaggaccca
     6121 gctggaggag ctggaagacg aactccaggc cacggaagat gccaagcttc gtctggaggt
     6181 caacatgcag gccatgaagg cgcagttcga gagagacctg caaaccaggg atgagcagaa
     6241 tgaagagaag aagcggctgc tgatcaaaca ggtgcgggag ctcgaggcgg agctggagga
     6301 tgagaggaaa cagcgggcgc ttgctgtagc ttcaaagaaa aagatggaga tagacctgaa
     6361 ggacctcgaa gcccaaatcg aggctgcgaa caaagctcgg gatgaggtga ttaagcagct
     6421 ccgcaagctc caggctcaga tgaaggatta ccaacgtgaa ttagaagaag ctcgtgcatc
     6481 cagagatgag atttttgctc aatccaaaga gagtgaaaag aaattgaaga gtctggaagc
     6541 agaaatcctt caattgcagg aggaacttgc ctcatctgag cgagcccgcc gacacgccga
     6601 gcaggagaga gatgagctgg cggacgagat caccaacagc gcctctggca agtccgcgct
     6661 gctggatgag aagcggcgtc tggaagctcg gatcgcacag ctggaggagg agctggaaga
     6721 ggagcagagc aacatggagc tgctcaacga ccgcttccgc aagaccactc tacaggtgga
     6781 cacactgaac gccgagctag cagccgagcg cagcgccgcc cagaagagtg acaatgcacg
     6841 ccagcaactg gagcggcaga acaaggagct gaaggccaag ctgcaggaac tcgagggtgc
     6901 tgtcaagtct aagttcaagg ccaccatctc agccctggag gccaagattg ggcagctgga
     6961 ggagcagctt gagcaggaag ccaaggaacg agcagccgcc aacaaattag tccgtcgcac
     7021 tgagaagaag ctgaaagaaa tcttcatgca ggttgaggat gagcgtcgac acgcggacca
     7081 gtataaagag cagatggaga aggccaacgc tcggatgaag cagcttaaac gccagctgga
     7141 ggaagcagaa gaagaagcga cgcgtgccaa cgcatctcgg cgtaaactcc agcgggaact
     7201 ggatgatgcc accgaggcca acgagggcct gagccgcgag gtcagcaccc tgaagaaccg
     7261 gctgaggcgg ggtggcccca tcagcttctc ttccagccga tctggccggc gccagctgca
     7321 ccttgaagga gcttccctgg agctctccga cgatgacaca gaaagtaaga ccagtgatgt
     7381 caacgagacg cagccacccc agtcagagta aggtaccgcg ggcccgggat ccaccggatc
     7441 tagataactg atca
//
