LOCUS       sequence-88887-e        6739 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 490 / 553, Emission: 507 / 
            573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6739)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..727
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg aacaccccgg gaattaacct
       61 gatcaaggag gacatgcgcg tgaaggtgca catggagggc aacgtgaacg gccacgcctt
      121 cgtgatcgag ggcgagggca agggcaagcc ctacgagggc acccagaccg ccaacctgac
      181 cgtgaaggag ggcgcccccc tgcccttcag ctacgacatc ctgaccaccg ccgtgcacta
      241 cggcaaccgg gtgttcacca agtaccccga ggacatcccc gactacttca agcagagctt
      301 ccccgagggc tacagctggg agcgcaccat gaccttcgag gacaagggca tctgcaccat
      361 ccgcagcgac atcagcctgg agggcgactg cttcttccag aacgtgcgct tcaagggcac
      421 caacttcccc cccaacggcc ccgtgatgca gaagaagacc ctgaagtggg agcccagcac
      481 cgagaagctg cacgtgcgcg acggcctgct ggtgggcaac atcaacatgg ccctgctgct
      541 ggagggcggc ggccactacc tgtgcgactt caagaccacc tacaaggcca agaaggtggt
      601 gcagctgccc gacgcccact tcgtggacca ccgcatcgag atcctgggca acgacagcga
      661 ctacaacaag gtgaagctgt acgagcacgc cgtggcccgc tacagccccc tgcccagcca
      721 ggtgtggtcc ggactcagat ctggcagcgg tggaggcagc gcatccggcg gaagcggaag
      781 cgctcagcag gctgcagaca agtacctcta tgtggataaa aacttcatca ataacccgct
      841 ggcccaagct gactgggctg ccaagaagtt ggtatgggtg ccttccagca agaatggctt
      901 tgaaccagcc agcctcaagg aggaggtggg agaagaggcc attgtagagc tggtagagaa
      961 tgggaagaag gtgaaggtga acaaggacga catccagaag atgaacccac ccaagttctc
     1021 caaggtggag gacatggcag agctcacgtg cctcaacgaa gcttcggtgc tgcacaacct
     1081 caaggagcga tactactcag ggcttatcta cacctattca ggcctgttct gtgtggtcat
     1141 caacccttat aagaacctgc ccatctactc agaggagatc gtggagatgt acaagggcaa
     1201 gaagaggcac gagatgccac cccacatcta cgccatcaca gatactgcct accggagcat
     1261 gatgcaggac cgggaagatc agtccatcct gtgcacgggg gagtctggag cagggaagac
     1321 agagaacacc aagaaagtca tccagtacct ggcacatgtg gcctcctcac acaagagcaa
     1381 gaaggaccag ggggagttgg agcggcagct gctacaggcc aaccctatcc tagaggcctt
     1441 tggaaacgcc aagacggtga agaatgacaa ctcctctcga ttcggtaaat tcattcgtat
     1501 caactttgat gtcaatggct acattgttgg tgccaacatt gagacttatc ttctggagaa
     1561 atctcgtgct atccgccaag ccaaagagga gcggaccttc cacatcttct actacctgct
     1621 gtctggggcc ggagaacacc tgaagactga tctcctgttg gagccataca acaaataccg
     1681 cttcctgtcc aacgggcacg tcaccatccc tgggcagcag gacaaggaca tgttccagga
     1741 gacaatggag gccatgagaa ttatgggtat cccagaggat gagcagatgg gcttgctgcg
     1801 ggtcatctct ggggtccttc agcttggcaa cattgccttc aagaaggagc ggaacactga
     1861 ccaggcgtcc atgccggaca acacagctgc tcaaaaggtg tcccacctcc tggggatcaa
     1921 tgtgaccgac ttcaccagag gcatcctcac cccacgcatc aaggtgggca gagactatgt
     1981 gcagaaggcg cagactaaag agcaggctga ctttgccatt gaggccttgg ccaaggctac
     2041 ctatgagcgg atgttccgct ggctggtgct tcgcatcaac aaagctctgg acaagaccaa
     2101 gaggcagggc gcctcattta tcgggatact ggacatcgct ggctttgaaa tctttgatct
     2161 gaactccttc gagcagctgt gcatcaacta caccaacgag aagctgcagc agctgttcaa
     2221 ccacaccatg ttcatcctgg agcaggagga gtaccagcga gagggcatcg agtggaactt
     2281 catcgacttc ggcctggacc tgcagccctg catcgacctc attgagaagc cggcgggtcc
     2341 cccaggcatc ctggccctgc tagatgagga gtgctggttt cctaaggcca ctgacaagag
     2401 cttcgtggag aaggtggtgc aggagcaggg cacccacccc aagttccaga agcccaagca
     2461 actgaaggac aaggctgatt tctgcattat ccactatgcc ggcaaggtgg actataaagc
     2521 tgacgagtgg ctgatgaaga acatggaccc cttgaacgac aacatcgcca cgctgcttca
     2581 ccagtcctca gacaagtttg tctctgagct gtggaaggat gtggatcgga tcattggctt
     2641 ggaccaagtg gctggaatgt ccgagacagc actacctggt gccttcaaga cccggaaggg
     2701 catgttccgt actgtcggac agctgtacaa ggagcagctg gccaagctca tggccacgtt
     2761 gaggaatacc aaccccaact tcgtgcgctg catcattccc aaccatgaga agaaggccgg
     2821 caaactggac ccgcacttgg tgctggacca gctgcgctgc aatggcgtcc ttgagggcat
     2881 ccggatctgc cgccagggct ttcccaacag ggtggtcttc caggagttcc ggcagaggta
     2941 tgagatcctc acccccaact ccatcccgaa gggcttcatg gatggcaagc aagcgtgtgt
     3001 gctcatgatc aaagccttgg agcttgacag caacctgtac cgcatcggcc agagcaaagt
     3061 gttcttccgg gcaggagtgc tcgcccacct ggaggaagag cgggacctga agatcaccga
     3121 tgtcatcatt ggcttccagg cctgctgcag gggctacctg gccaggaagg cctttgccaa
     3181 gaggcagcaa cagctgaccg ccatgaaggt cctacagagg aactgtgctg cgtacctcag
     3241 gctgcgcaac tggcagtggt ggaggctctt caccaaggtc aagcccctgt tgaactcaat
     3301 aagacatgag gatgagctgt tagccaagga ggcggaactg acaaaggttc gagagaaaca
     3361 tctggctgca gagaacaggc tgacagagat ggagacgatg cagtctcagc tcatggcaga
     3421 gaagctgcag cttcaggagc agctgcaggc ggagacagag ctgtgtgccg aggctgagga
     3481 gctccgggcc cgtctgacag cgaagaagca ggagctggag gagatatgcc atgacctgga
     3541 ggccagggtg gaggaggagg aggagcgctg ccagtacctg caggccgaga agaagaagat
     3601 gcagcagaac atccaggaac ttgaggagca gttggaggag gaggagagcg cccggcagaa
     3661 gctgcagctt gagaaggtga ccaccgaggc caagctgaag aaactggagg aggaccagat
     3721 catcatggag gaccagaact gcaaactggc caaggagaag aaactgctgg aagacagagt
     3781 agctgagttc actaccaacc tcatggaaga ggaggagaag tccaagagcc tggccaagct
     3841 caagaacaag cacgaggcaa tgatcaccga cctggaagag cgcctccgta gggaggagaa
     3901 gcagaggcag gagttggaga agacccgtcg caagctggag ggagactcca cagacctcag
     3961 tgaccagatt gctgagctcc aggcgcagat agcagagctc aagatgcagc tggccaagaa
     4021 ggaggaggag ttgcaggctg ccttggccag agtggaagaa gaagctgctc agaagaatat
     4081 ggccctgaag aagatccgag aactggaaac tcagatatct gagctccagg aggacctgga
     4141 gtcggagcga gcctccagga ataaagccga gaagcagaaa cgggatctgg gagaggagct
     4201 ggaggcgctg aagacagagc tggaggacac gctggactcc acggctgccc agcaggagct
     4261 gaggtcgaag cgtgagcagg aggtgagcat cctgaagaag actctggagg acgaggccaa
     4321 gacccatgag gcccagatcc aggagatgag gcagaagcac tcacaggctg tggaggagct
     4381 ggcagatcag ttggagcaga cgaagcgggt aaaagctacc cttgagaagg cgaagcagac
     4441 cctggagaat gagcggggag agctggccaa tgaggtgaag gccctgctgc aaggcaaggg
     4501 cgactcagag cacaagcgca agaaggtgga ggcgcagctg caagaactgc aggtcaagtt
     4561 cagcgaggga gagcgcgtgc gaaccgaact ggccgacaag gtcaccaagc tgcaggttga
     4621 actggacagt gtgaccggtc tccttagcca gtctgacagc aagtccagca agcttacgaa
     4681 ggacttctct gcgctggagt cccagcttca ggacacacag gagttgctcc aggaggagaa
     4741 ccggcagaag ctgagcctga gcaccaagct caagcagatg gaggatgaga aaaactcctt
     4801 cagggagcag ctggaggagg aggaggaggc caagcgcaac ttggagaagc agatcgccac
     4861 gctccatgcc caggtgaccg acatgaagaa gaagatggag gacggtgtag ggtgcctgga
     4921 gactgcagag gaggcgaagc ggaggcttca gaaggacttg gaaggcctga gccagcggct
     4981 tgaggagaag gtggctgcct acgataagct ggagaagacc aagacacggc tgcagcagga
     5041 gctggacgac ctgctggttg acctggacca ccagcggcag agcgtctcca acctggaaaa
     5101 gaagcagaag aagttcgacc agctcctagc cgaggagaag accatctcgg ccaagtatgc
     5161 agaggagcgt gaccgagctg aggctgaggc ccgtgagaag gagacaaagg cgctatcact
     5221 ggcccgggcg cttgaggagg ccatggagca gaaggcagag ctggagcggc tcaacaagca
     5281 gttccgcacg gagatggagg acctcatgag ctccaaggat gacgtgggca agagtgtcca
     5341 cgagctggag aagtccaagc gggccttgga gcagcaggtg gaggagatga agacccagct
     5401 ggaggagctg gaggatgagc tgcaggccac ggaggatgcc aagctccgcc tggaggtgaa
     5461 cctgcaggcc atgaaggccc agtttgagcg ggatctgcag ggccgggatg aacagagcga
     5521 ggagaagaag aagcagctgg tcagacaggt gcgggagatg gaggcggagc tggaggatga
     5581 gaggaagcag cgctccatgg ccatggccgc acgcaagaaa ctggagatgg atctgaagga
     5641 cctggaggca cacattgaca cagccaataa gaaccgggaa gaggccatca aacagctgcg
     5701 gaagcttcag gcccagatga aggactgcat gcgggagctg gacgacacgc gcgcctcccg
     5761 ggaggagatc ctggcgcagg ccaaggagaa tgagaagaag ctgaagagca tggaggccga
     5821 gatgattcag ctgcaggagg aactggcagc tgctgagcgt gctaagcgtc aggcccaaca
     5881 ggaacgggac gagctggctg atgagatcgc caacagcagt ggcaaagggg ccctagcatt
     5941 agaggagaag cggcgactgg aggcccgcat tgccctgctg gaggaggagc tggaggagga
     6001 acagggcaac acggagctga tcaacgatcg gctgaagaag gccaacctgc agatcgacca
     6061 aataaacacc gacctgaacc tggaacgcag ccacgcacag aagaatgaga atgcgcgaca
     6121 gcagctggaa cgccagaaca aggagctcaa ggccaagctg caggaaatgg agagtgctgt
     6181 caagtccaaa tacaaggcct ccatcgcggc cttggaggcc aaaattgcac agctggagga
     6241 acagctggac aacgagacca aggagcgcca ggcagcctcc aagcaggtgc gccggacgga
     6301 gaagaagctg aaggacgtgc tgctgcaggt ggaggacgag cggaggaacg cggaacagtt
     6361 caaggaccag gctgacaagg cgtccacccg cctgaagcag cttaaacggc agctagagga
     6421 ggctgaagag gaggcccagc gggccaatgc ctcacgccgg aagctgcagc gtgagctgga
     6481 agatgccaca gagaccgctg atgctatgaa ccgcgaggtc agctccctga agaacaaact
     6541 gaggcgtggg gacctgccat ttgtcgtgac tcgccgaatt gttcggaaag gcactggcga
     6601 ctgctcagac gaggaggtcg acggtaaagc agatggggcc gatgccaagg cagctgaata
     6661 ggaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
     6721 taatcagcca taccacatt
//
