LOCUS       sequence-89038-e        6700 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6700)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..691
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgagtgtga ttaaaccaga
       61 gatgaagatg aggtactaca tggacggctc cgtcaatggg catgagttca caattgaagg
      121 tgaaggcaca ggcagacctt acgagggaca tcaagagatg acactacgcg tcacaatggc
      181 cgagggcggg ccaatgcctt tcgcgtttga cttagtgtca cacgtgttct gttacggcca
      241 cagagtattt actaaatatc cagaagagat accagactat ttcaaacaag catttcctga
      301 aggcctgtca tgggaaaggt cgttggagtt cgaagatggt gggtccgctt cagtcagtgc
      361 gcatataagc cttagaggaa acaccttcta ccacaaatcc aaatttactg gggttaactt
      421 tcctgccgat ggtcctatca tgcaaaacca aagtgttgat tgggagccat caaccgagaa
      481 aattactgcc agcgacggag ttctgaaggg tgatgttacg atgtacctaa aacttgaagg
      541 aggcggcaat cacaaatgcc aaatgaagac tacttacaag gcggcaaaag agattcttga
      601 aatgccagga gaccattaca tcggccatcg cctcgtcagg aaaaccgaag gcaacattac
      661 tgagcaggta gaagatgcag tagctcatag ctccggactc agatctggca gcggtggagg
      721 cagcgcatcc ggcggaagcg gaagcgctca gcaggctgca gacaagtacc tctatgtgga
      781 taaaaacttc atcaataacc cgctggccca agctgactgg gctgccaaga agttggtatg
      841 ggtgccttcc agcaagaatg gctttgaacc agccagcctc aaggaggagg tgggagaaga
      901 ggccattgta gagctggtag agaatgggaa gaaggtgaag gtgaacaagg acgacatcca
      961 gaagatgaac ccacccaagt tctccaaggt ggaggacatg gcagagctca cgtgcctcaa
     1021 cgaagcttcg gtgctgcaca acctcaagga gcgatactac tcagggctta tctacaccta
     1081 ttcaggcctg ttctgtgtgg tcatcaaccc ttataagaac ctgcccatct actcagagga
     1141 gatcgtggag atgtacaagg gcaagaagag gcacgagatg ccaccccaca tctacgccat
     1201 cacagatact gcctaccgga gcatgatgca ggaccgggaa gatcagtcca tcctgtgcac
     1261 gggggagtct ggagcaggga agacagagaa caccaagaaa gtcatccagt acctggcaca
     1321 tgtggcctcc tcacacaaga gcaagaagga ccagggggag ttggagcggc agctgctaca
     1381 ggccaaccct atcctagagg cctttggaaa cgccaagacg gtgaagaatg acaactcctc
     1441 tcgattcggt aaattcattc gtatcaactt tgatgtcaat ggctacattg ttggtgccaa
     1501 cattgagact tatcttctgg agaaatctcg tgctatccgc caagccaaag aggagcggac
     1561 cttccacatc ttctactacc tgctgtctgg ggccggagaa cacctgaaga ctgatctcct
     1621 gttggagcca tacaacaaat accgcttcct gtccaacggg cacgtcacca tccctgggca
     1681 gcaggacaag gacatgttcc aggagacaat ggaggccatg agaattatgg gtatcccaga
     1741 ggatgagcag atgggcttgc tgcgggtcat ctctggggtc cttcagcttg gcaacattgc
     1801 cttcaagaag gagcggaaca ctgaccaggc gtccatgccg gacaacacag ctgctcaaaa
     1861 ggtgtcccac ctcctgggga tcaatgtgac cgacttcacc agaggcatcc tcaccccacg
     1921 catcaaggtg ggcagagact atgtgcagaa ggcgcagact aaagagcagg ctgactttgc
     1981 cattgaggcc ttggccaagg ctacctatga gcggatgttc cgctggctgg tgcttcgcat
     2041 caacaaagct ctggacaaga ccaagaggca gggcgcctca tttatcggga tactggacat
     2101 cgctggcttt gaaatctttg atctgaactc cttcgagcag ctgtgcatca actacaccaa
     2161 cgagaagctg cagcagctgt tcaaccacac catgttcatc ctggagcagg aggagtacca
     2221 gcgagagggc atcgagtgga acttcatcga cttcggcctg gacctgcagc cctgcatcga
     2281 cctcattgag aagccggcgg gtcccccagg catcctggcc ctgctagatg aggagtgctg
     2341 gtttcctaag gccactgaca agagcttcgt ggagaaggtg gtgcaggagc agggcaccca
     2401 ccccaagttc cagaagccca agcaactgaa ggacaaggct gatttctgca ttatccacta
     2461 tgccggcaag gtggactata aagctgacga gtggctgatg aagaacatgg accccttgaa
     2521 cgacaacatc gccacgctgc ttcaccagtc ctcagacaag tttgtctctg agctgtggaa
     2581 ggatgtggat cggatcattg gcttggacca agtggctgga atgtccgaga cagcactacc
     2641 tggtgccttc aagacccgga agggcatgtt ccgtactgtc ggacagctgt acaaggagca
     2701 gctggccaag ctcatggcca cgttgaggaa taccaacccc aacttcgtgc gctgcatcat
     2761 tcccaaccat gagaagaagg ccggcaaact ggacccgcac ttggtgctgg accagctgcg
     2821 ctgcaatggc gtccttgagg gcatccggat ctgccgccag ggctttccca acagggtggt
     2881 cttccaggag ttccggcaga ggtatgagat cctcaccccc aactccatcc cgaagggctt
     2941 catggatggc aagcaagcgt gtgtgctcat gatcaaagcc ttggagcttg acagcaacct
     3001 gtaccgcatc ggccagagca aagtgttctt ccgggcagga gtgctcgccc acctggagga
     3061 agagcgggac ctgaagatca ccgatgtcat cattggcttc caggcctgct gcaggggcta
     3121 cctggccagg aaggcctttg ccaagaggca gcaacagctg accgccatga aggtcctaca
     3181 gaggaactgt gctgcgtacc tcaggctgcg caactggcag tggtggaggc tcttcaccaa
     3241 ggtcaagccc ctgttgaact caataagaca tgaggatgag ctgttagcca aggaggcgga
     3301 actgacaaag gttcgagaga aacatctggc tgcagagaac aggctgacag agatggagac
     3361 gatgcagtct cagctcatgg cagagaagct gcagcttcag gagcagctgc aggcggagac
     3421 agagctgtgt gccgaggctg aggagctccg ggcccgtctg acagcgaaga agcaggagct
     3481 ggaggagata tgccatgacc tggaggccag ggtggaggag gaggaggagc gctgccagta
     3541 cctgcaggcc gagaagaaga agatgcagca gaacatccag gaacttgagg agcagttgga
     3601 ggaggaggag agcgcccggc agaagctgca gcttgagaag gtgaccaccg aggccaagct
     3661 gaagaaactg gaggaggacc agatcatcat ggaggaccag aactgcaaac tggccaagga
     3721 gaagaaactg ctggaagaca gagtagctga gttcactacc aacctcatgg aagaggagga
     3781 gaagtccaag agcctggcca agctcaagaa caagcacgag gcaatgatca ccgacctgga
     3841 agagcgcctc cgtagggagg agaagcagag gcaggagttg gagaagaccc gtcgcaagct
     3901 ggagggagac tccacagacc tcagtgacca gattgctgag ctccaggcgc agatagcaga
     3961 gctcaagatg cagctggcca agaaggagga ggagttgcag gctgccttgg ccagagtgga
     4021 agaagaagct gctcagaaga atatggccct gaagaagatc cgagaactgg aaactcagat
     4081 atctgagctc caggaggacc tggagtcgga gcgagcctcc aggaataaag ccgagaagca
     4141 gaaacgggat ctgggagagg agctggaggc gctgaagaca gagctggagg acacgctgga
     4201 ctccacggct gcccagcagg agctgaggtc gaagcgtgag caggaggtga gcatcctgaa
     4261 gaagactctg gaggacgagg ccaagaccca tgaggcccag atccaggaga tgaggcagaa
     4321 gcactcacag gctgtggagg agctggcaga tcagttggag cagacgaagc gggtaaaagc
     4381 tacccttgag aaggcgaagc agaccctgga gaatgagcgg ggagagctgg ccaatgaggt
     4441 gaaggccctg ctgcaaggca agggcgactc agagcacaag cgcaagaagg tggaggcgca
     4501 gctgcaagaa ctgcaggtca agttcagcga gggagagcgc gtgcgaaccg aactggccga
     4561 caaggtcacc aagctgcagg ttgaactgga cagtgtgacc ggtctcctta gccagtctga
     4621 cagcaagtcc agcaagctta cgaaggactt ctctgcgctg gagtcccagc ttcaggacac
     4681 acaggagttg ctccaggagg agaaccggca gaagctgagc ctgagcacca agctcaagca
     4741 gatggaggat gagaaaaact ccttcaggga gcagctggag gaggaggagg aggccaagcg
     4801 caacttggag aagcagatcg ccacgctcca tgcccaggtg accgacatga agaagaagat
     4861 ggaggacggt gtagggtgcc tggagactgc agaggaggcg aagcggaggc ttcagaagga
     4921 cttggaaggc ctgagccagc ggcttgagga gaaggtggct gcctacgata agctggagaa
     4981 gaccaagaca cggctgcagc aggagctgga cgacctgctg gttgacctgg accaccagcg
     5041 gcagagcgtc tccaacctgg aaaagaagca gaagaagttc gaccagctcc tagccgagga
     5101 gaagaccatc tcggccaagt atgcagagga gcgtgaccga gctgaggctg aggcccgtga
     5161 gaaggagaca aaggcgctat cactggcccg ggcgcttgag gaggccatgg agcagaaggc
     5221 agagctggag cggctcaaca agcagttccg cacggagatg gaggacctca tgagctccaa
     5281 ggatgacgtg ggcaagagtg tccacgagct ggagaagtcc aagcgggcct tggagcagca
     5341 ggtggaggag atgaagaccc agctggagga gctggaggat gagctgcagg ccacggagga
     5401 tgccaagctc cgcctggagg tgaacctgca ggccatgaag gcccagtttg agcgggatct
     5461 gcagggccgg gatgaacaga gcgaggagaa gaagaagcag ctggtcagac aggtgcggga
     5521 gatggaggcg gagctggagg atgagaggaa gcagcgctcc atggccatgg ccgcacgcaa
     5581 gaaactggag atggatctga aggacctgga ggcacacatt gacacagcca ataagaaccg
     5641 ggaagaggcc atcaaacagc tgcggaagct tcaggcccag atgaaggact gcatgcggga
     5701 gctggacgac acgcgcgcct cccgggagga gatcctggcg caggccaagg agaatgagaa
     5761 gaagctgaag agcatggagg ccgagatgat tcagctgcag gaggaactgg cagctgctga
     5821 gcgtgctaag cgtcaggccc aacaggaacg ggacgagctg gctgatgaga tcgccaacag
     5881 cagtggcaaa ggggccctag cattagagga gaagcggcga ctggaggccc gcattgccct
     5941 gctggaggag gagctggagg aggaacaggg caacacggag ctgatcaacg atcggctgaa
     6001 gaaggccaac ctgcagatcg accaaataaa caccgacctg aacctggaac gcagccacgc
     6061 acagaagaat gagaatgcgc gacagcagct ggaacgccag aacaaggagc tcaaggccaa
     6121 gctgcaggaa atggagagtg ctgtcaagtc caaatacaag gcctccatcg cggccttgga
     6181 ggccaaaatt gcacagctgg aggaacagct ggacaacgag accaaggagc gccaggcagc
     6241 ctccaagcag gtgcgccgga cggagaagaa gctgaaggac gtgctgctgc aggtggagga
     6301 cgagcggagg aacgcggaac agttcaagga ccaggctgac aaggcgtcca cccgcctgaa
     6361 gcagcttaaa cggcagctag aggaggctga agaggaggcc cagcgggcca atgcctcacg
     6421 ccggaagctg cagcgtgagc tggaagatgc cacagagacc gctgatgcta tgaaccgcga
     6481 ggtcagctcc ctgaagaaca aactgaggcg tggggacctg ccatttgtcg tgactcgccg
     6541 aattgttcgg aaaggcactg gcgactgctc agacgaggag gtcgacggta aagcagatgg
     6601 ggccgatgcc aaggcagctg aataggaatt ctgcagtcga cggtaccgcg ggcccgggat
     6661 ccaccggatc tagataactg atcataatca gccataccac
//
