LOCUS       sequence-89081-e         998 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             282..932
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatggtg agcaagggcg aggagaataa catggccgtg attaaaccag
      301 agatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag
      361 gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg
      421 ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc
      481 acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg
      541 aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg
      601 cgcatataag ccttagagga aacaccttct accacaaatc caaatttact ggggttaact
      661 ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga
      721 aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag
      781 gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa aagattctta
      841 aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta
      901 ctgagctggt agaagatgca gtagctcatt ccggcggcat ggacgagctg tacaagtaag
      961 cggccgcgac tctagatcat aatcagccat accacatt
//
