LOCUS       sequence-89104-e         994 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     30..50
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             179..886
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca ccatgctgct
      121 atccgtgccg ttgctgctcg gcctcctcgg cctggccgtc gccgcaccgg tcgccaccat
      181 ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt
      241 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      301 cccctacgag ggctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt
      361 cgcctgggac atcctgtccc ctcatttcac ctacggctcc aaggcctacg tgaagcaccc
      421 cgccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      481 gatgaactac gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      541 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtgat
      601 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggtgc
      661 cctgaagggc aagatcaaga tgaggctgaa gctgaaggac ggcggccact acacctccga
      721 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga
      781 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      841 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gaaaggacga
      901 gctgtaagaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac
      961 tgatcataat cagccatacc acatttgtag aggt
//
