LOCUS       sequence-89223-e        2886 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Gap Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: tdTomato-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1407..1416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1413..2843
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcggca
       61 gcacttttct ttcattgggg ggaaggcgtg aggaaagtac caaacagcag cagactttta
      121 aactttaaac agacaggtct gagagcctga actctcattt ttcctttgac ttcagcctcc
      181 aaggagttcc accaactttg gcgcgccggc ttcactttca ttaagtgaaa gagaggtgcc
      241 cagacatggg tgactggagt gccttgggga agcttctgga caaggtccaa gcctactcca
      301 ccgctggagg gaaggtgtgg ctgtcagtgc tcttcatatt cagaatcctg ctcctgggga
      361 cagctgttga gtcagcttgg ggtgatgaac agtctgcctt tcgctgtaac actcaacaac
      421 ctggctgcga aaacgtctgc tatgacaagt ccttccccat ctctcacgtg cgcttctggg
      481 tccttcagat catattcgtg tctgtgccca cactcctgta cttggcccat gtgttctatg
      541 tgatgaggaa ggaagagaag ctaaacaaga aagaagagga gctcaaagtg gcccagactg
      601 acggggtcaa cgtggagatg cacctgaagc agattgaaat caagaagttc aagtacggga
      661 ttgaagagca cggcaaggtg aaaatgaggg gcggcttgct gagaacctac atcatcagca
      721 tcctcttcaa gtctgtcttc gaggtggcct tcctgctcat ccagtggtac atctatgggt
      781 tcagcttgag cgcggtctac acctgcaaga gagatccctg cccccaccag gtagactgct
      841 tcctctcacg tcccacggag aaaaccatct tcatcatctt catgctggtg gtgtccttgg
      901 tgtctctcgc tttgaacatc attgagctct tctacgtctt cttcaaaggc gttaaggatc
      961 gcgtgaaggg aagaagcgat ccttaccacg ccaccactgg cccactgagc ccatcaaaag
     1021 actgcggatc tccaaaatac gcctacttca atggctgctc ctcaccaacg gctccactct
     1081 cgcctatgtc tcctcctggg tacaagctgg ttactggtga cagaaacaat tcctcgtgcc
     1141 gcaattacaa caagcaagct agcgagcaaa actgggcgaa ctacagcgca gagcaaaatc
     1201 gcatggggca ggccggaagc accatctcca actcgcacgc ccagccgttc gatttccccg
     1261 acgacaacca gaatgccaaa aaagttgctg ctggacatga actccagcca ttagccatcg
     1321 tggaccaacg accttccagc agagccagca gccgcgccag cagcaggcct cggcctgatg
     1381 acctggagat tgcggatcca ccggtcgcca ccatggtgag caagggcgag gaggtcatca
     1441 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     1501 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1561 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     1621 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1681 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1741 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1801 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1861 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1921 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1981 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     2041 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     2101 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     2161 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     2221 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2281 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     2341 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     2401 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     2461 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     2521 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     2581 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     2641 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     2701 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     2761 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     2821 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     2881 tgagag
//
