LOCUS       sequence-89238-e        1907 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1907)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..1454
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             1485..1862
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
       61 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      241 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      361 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
      481 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
      541 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
      601 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
      721 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
      781 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      841 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      901 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
      961 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1021 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1081 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1141 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1201 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1261 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1321 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1381 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     1441 acgagctgta caagtccgga ctcagatctg gaggctccgg aggcccagag ccagcgaagt
     1501 ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg
     1561 gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga
     1621 agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg
     1681 tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc
     1741 gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt
     1801 tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagt
     1861 aaggatccac cggatctaga taactgatca taatcagcca taccaca
//
