LOCUS       sequence-89246-e        1814 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1814)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             384..1814
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gatccgctag cgccaccatg aagttaagtc gccagttcac cgtgtttggc agcgcgatct
       61 tctgcgtcgt aatcttctca ctctacctga tgctggacag gggtcacttg gactaccctc
      121 ggggcccgcg ccaggagggc tcctttccgc agggccagct ttcaatattg caagaaaaga
      181 ttgaccattt ggagcgtttg ctcgctgaga acaacgagat catctcaaat atcagagact
      241 cagtcatcaa cctgagcgag tctgtggagg acggcccgcg ggggtcacca ggcaacgcca
      301 gccaaggctc catccacctc cactcgccac agttggccct gcaggctgac cccagctccg
      361 gatcggatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca
      421 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      481 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      541 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
      601 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
      661 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
      721 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
      781 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
      841 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
      901 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
      961 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     1021 aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca
     1081 ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag
     1141 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     1201 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1261 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     1321 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     1381 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     1441 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     1501 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     1561 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     1621 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     1681 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     1741 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     1801 agctgtacaa gtaa
//
