LOCUS       sequence-89267-e        2850 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Cilium, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2850)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1381..2811
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatggc cactgttacc
       61 tatccttcat ctgagcctac gaccttggac cctgggaacg catcctcgac ctggcccctg
      121 gataccaccc tggggaacac atccgctggc gctagcctga caggcctggc tgtcagtggc
      181 atcttgatct ctctggtgta cctggtggtg tgcgtggtgg gtttgctggg caactcgctg
      241 gtgatctacg tggtcctgcg gcacacgtcc agcccatcag tgaccagtgt ctatatcctc
      301 aacctggctc tggctgatga gctcttcatg ctagggctac ccttcctggc tgctcagaac
      361 gccctgtcct actggccctt cggatctctc atgtgccgtc tggtcatggc cgtggatggc
      421 atcaaccagt tcaccagcat cttctgcctc accgtcatga gtgtggaccg ctatctggct
      481 gtggtgcacc ccacacgctc agcccgctgg cgcacggcac cagtggctcg cacggtcagt
      541 gcagctgtct gggtggcctc ggctgtggtg gtgctgcctg tggttgtgtt ctcaggagtg
      601 ccccggggca tgagcacgtg ccacatgcag tggccagagc cagcggctgc ctggcgaaca
      661 gccttcatca tctacacggc cgcactgggc ttctttgggc ccctgctggt catctgcttg
      721 tgctacttgc tcattgtggt aaaggtgcgg tcgaccaccc ggcgggtgcg ggcgccctcg
      781 tgtcagtggg tacaggcacc cgcatgccag cggcgacgcc gctctgagcg cagggtcaca
      841 cgcatggtgg tggccgtggt ggcactcttc gtcctctgct ggatgccttt ctatctgctc
      901 aacatcgtca atgtggtgtg cccgctgccg gaggagcccg ccttcttcgg cctctacttc
      961 ctggtggtgg cgctgcccta cgccaacagc tgcgcaaacc ccatcctcta cggcttcctc
     1021 tcctaccgct tcaagcaggg ctttcgcagg atcctgctaa gaccatcacg tcgcattcgg
     1081 agccaggagc cagggtcggg acctccagag aagactgaag aggaggagga tgaagaagaa
     1141 gaagagagaa gggaagagga ggagcggagg atgcagagag ggcaggagat gaacgggagg
     1201 ctcagtcaga tcgcacaggc tggcactagt ggacaacagc cacggccctg cacagggact
     1261 gctaaggagc agcagcttct gccccaggag gccacagctg gggacaaggc cagcacactg
     1321 agccatctgg gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
     1381 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     1441 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1501 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1561 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     1621 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1681 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     1741 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1801 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     1861 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     1921 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     1981 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     2041 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
     2101 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
     2161 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     2221 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     2281 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     2341 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2401 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     2461 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     2521 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     2581 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     2641 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     2701 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     2761 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta agcggccgcg
     2821 actctagatc ataatcagcc ataccacatt
//
