LOCUS       sequence-89513-e        1013 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  cRNA probe to detect the slc6a3 transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Bai Q, Burton EA
  TITLE     Cis-acting elements responsible for dopaminergic neuron-specific 
            expression of zebrafish slc6a3 (dopamine transporter) in vivo are 
            located remote from the transcriptional start site.
  JOURNAL   Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 
            10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12.
  PUBMED    19755139
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuroscience.2009.09.014"; journalName: "Neuroscience"; 
            date: "2009-12-15- 15"; volume: "164"; issue: "3"; pages: "1138-51"
COMMENT     Sequence Label: slc6a3 probe
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(768..784)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(768..784)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(821..841)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(821..839)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(860..876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(860..876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(873..895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    884..900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(908..914,915..932,933..938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    953..974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattctaca tggagctggc gctgggtcaa tataaccgtg aaggagccgc
      121 cggcgtctgg aagatctgcc ccatattcaa aggcgtgggc ttcacggtga tcctgatctc
      181 gctgtatgtg ggcttctact acaacgtgat catcgcctgg gctctgtttt acctgttctc
      241 ctccttctcc ggggagctgc cctggatcca ctgcaacaac acctggaaca gcccaaactg
      301 ctccgacctc aacgccacgc tgctcaacga cacgtacaag accacgcccg cgctcgagta
      361 cttcgagcgg ggtgttctgc atgttcatga aagctctggt attgatgatc tgggtgcgcc
      421 gcgctggcag ctgaccgcct gtctggccgt ggtcatcgtg gtcctctact tcagcctctg
      481 gaaaggagtg aagacgtctg ggaaggtggt gtggatcact gccacgatgc cgtatgtggt
      541 cctgaccgtg ctgctcctgc gtggcgtcac tctgcctgga gccatcgacg gcattaaagc
      601 ttacctgagt gtggacttcc tgcgcctgta tgatgctcag gtctggatcg aggccgccac
      661 gcagatctgc ttttctctgg gagtcgggtt tggtgtgcta atcgccttct ccagctacaa
      721 caaattcagc aacaactgct acagagacga attcctgcag cccgggggat ccactagttc
      781 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
      841 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aac
//
