LOCUS       sequence-89592-e        1032 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  TITLE     Karl Kramer plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BirA(R118G)-HA destination vector
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(321..340)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(322..340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(385..588)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(414..434)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        589..969
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgttgataa caggccttaa
      301 gcgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      361 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      421 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      481 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      541 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      601 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
      661 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
      721 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc cattgacgtc
      781 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
      841 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
      901 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
      961 atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     1021 taagcagatt ct
//
