LOCUS       sequence-89646-e       11528 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-FP-GFP
FEATURES             Location/Qualifiers
     source          1..11528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             95..328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(353..371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(661..680)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(893..912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1012..1034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1072..1090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1158..1262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1263..1331,1332..2123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1481..1500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3036..3053
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3123..3143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3128..3457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3308..3443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4591..4656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4786..4806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5231..5365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5268..5287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5322..5341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5534..6167
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6214..6339
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6832..7065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7250..7294
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7579..7696
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7773..7897
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7922..7927,7928..7945,7946..7952)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8006..8665
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8072..8091)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9007..9312
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9191..9210
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9353..9477)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9596..10003
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      10019..10028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10025..10027,10028..10030,10031..10744)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10025..10027,10028..10030,10031..10741)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10070..10091)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10331..10350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10678..10699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10925..11513
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10978..10998)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11396..11407)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(11516..11528,1..4))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(11517..11528,1..5))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
       61 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
      121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
      181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      301 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
      361 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
      421 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
      481 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
      541 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
      601 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      661 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      721 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
      781 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaactac tactgggctg
      841 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
      901 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      961 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1021 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1081 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1261 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1321 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1381 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1441 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1501 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1561 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1621 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1681 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1741 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1801 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1861 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1921 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1981 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2041 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2101 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2161 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2221 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2461 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2941 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3001 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3061 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     3481 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     3541 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     3601 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3661 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3721 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     3781 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3841 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3901 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     3961 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     4021 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     4081 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     4141 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     4201 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     4261 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     4321 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     4381 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     4441 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     4501 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     4561 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     4621 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     4681 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4741 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     4801 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     4861 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     4921 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     4981 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     5041 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     5101 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     5161 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5221 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     5401 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
     5461 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     5521 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     5581 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     5641 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     5701 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     5761 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     5821 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     5881 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     5941 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     6001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6181 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6241 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6301 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6361 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6421 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     6481 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     6541 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     6601 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     6661 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagatn nnagggacaa
     6721 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     6781 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     6841 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     6901 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     6961 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7021 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7141 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7201 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7261 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7321 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7381 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7441 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7501 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     7561 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     7621 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     7681 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     7741 gcttgatatc gaattatcac aagtttgtaa tcacaagttt gtacaaaaaa gctgaacgag
     7801 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     7861 cataatactg taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg
     7921 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
     7981 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     8041 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     8101 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     8161 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     8221 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     8281 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     8341 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     8401 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     8461 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     8521 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     8581 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     8641 cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca
     8701 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     8761 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     8821 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
     8881 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
     8941 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct
     9001 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     9061 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
     9121 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     9181 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     9241 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     9301 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
     9361 ctggatatgt tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa
     9421 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat
     9481 tacaaagtgg tgatttaaaa ttcctgcagc cccgataaaa taaaagattt tatttagtct
     9541 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag ctgcagtaac
     9601 gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc
     9661 gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc
     9721 cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga
     9781 acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt
     9841 ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct
     9901 gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc
     9961 ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc caccggtcgc
    10021 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
    10081 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
    10141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
    10201 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
    10261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
    10321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
    10381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
    10441 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
    10501 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
    10561 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
    10621 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
    10681 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
    10741 gtaaanngcg gccgcgactc tagagtcgac tgcagcatgn nnnaagcttg atatcaagct
    10801 tatcgatnna atggtggggc aatcatctac atttcatggg atatgtgatt actagttcag
    10861 gtgtattgcc acaagacaaa catgttaaga aaatttcccg ttatttgcac tcnnaattcc
    10921 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10981 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    11041 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    11101 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    11161 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    11221 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    11281 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
    11341 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    11401 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    11461 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
    11521 ccgtcgac
//
