LOCUS       sequence-89701-e         127 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Retroviral vector for expressing Neuroserpin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 127)
  AUTHORS   Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, 
            Kris MG, Huse JT, Brogi E, Massague J
  TITLE     Serpins promote cancer cell survival and vascular co-option in brain
            metastasis.
  JOURNAL   Cell. 2014 Feb 27;156(5):1002-16. doi: 10.1016/j.cell.2014.01.040.
  PUBMED    24581498
REFERENCE   2  (bases 1 to 127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-27- 
            27"; volume: "156"; issue: "5"; pages: "1002-16"
COMMENT     Sequence Label: pBabe-FLAG-Neuroserpin
FEATURES             Location/Qualifiers
     source          1..127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..33
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg gccggatcca
       61 ccatggacta caaagacgat gacgacaaga tggctttcct tggactcttc tcttgnnntn
      121 nnannng
//
