LOCUS       sequence-89755-e         891 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 584, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: JRed-C1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..52,53..760)
                     /codon_start=1
                     /product="red fluorescent protein KillerRed, a 
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /label=KillerRed
                     /note="mammalian codon-optimized"
                     /translation="GSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNV
                     HAVCETGKLPMSWKPICHLIQYGEPFFARYPNGISHFAQECFPEGLSIDRTVRFENDGT
                     MTSHHTYELDGTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLAF
                     IGFTTADGGLMMGHFDSKMTFNGSRAIKIPGPHFVTIITKQMRDTSDKRDHVCQREVTY
                     AHSVPRITSAIGSDED"
     misc_feature    761..826
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggac gaggatggtt cagagggcgg
       61 ccccgccctg ttccagagcg acatgacctt caaaatcttc atcgacggcg aggtgaacgg
      121 ccagaagttc accatcgtgg ccgacggcag cagcaagttc ccccacggcg acttcaacgt
      181 gcacgccgtg tgcgagaccg gcaagctgcc catgagctgg aagcccatct gccacctgat
      241 ccagtacggc gagcccttct tcgcccgcta ccccaacggc atcagccact tcgcccagga
      301 gtgcttcccc gagggcctga gcatcgaccg caccgtgcgc ttcgagaacg acggcaccat
      361 gaccagccac cacacctacg agctggacgg cacctgcgtg gtcagccgca tcaccgtgaa
      421 ctgcgacggc ttccagcccg acggccccat catgcgcgac cagctggtgg acatcctgcc
      481 caacgagacc cacatgttcc cccacggccc caacgccgtg cgccagctgg ccttcatcgg
      541 cttcaccacc gccgacggcg gcctgatgat gggccacttc gacagcaaga tgaccttcaa
      601 cggcagccgc gccatcaaga tccccggccc ccacttcgtg accatcatca ccaagcagat
      661 gagggacacc agcgacaagc gcgaccacgt gtgccagcgc gaggtgacct acgcccacag
      721 cgtgccccgc atcaccagcg ccatcggtag cgacgaggat tccggactca gatctcgagc
      781 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
      841 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa a
//
