LOCUS       sequence-90046-e        1141 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TwinStrepII Prescission SNAPf TEV clo...
FEATURES             Location/Qualifiers
     source          1..1141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     602..619
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             729..752
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             789..812
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             813..836
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      241 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      301 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      361 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      421 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      481 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      541 tggctacgta tactccggac tattaataga tcatggagat aattagcggc cgcttaatta
      601 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
      661 taaaaaaacc tataactatt ccggattatt cataccgtcc caccatcggg cgcggatcca
      721 tggcttcatg gtctcacccc caatttgaga aaggcggtgg cagcggcggc ggtagcggag
      781 gtggcagctg gtcacaccca caattcgaga aattggaggt actgttccag ggcccgggaa
      841 gcggcgataa agactgcgag atgaagcgca cgacgctcga ttcgcccctt ggcaaattgg
      901 aactgagcgg ttgtgagcaa ggtctgcatc gtatcatctt tctgggaaag ggaactagtg
      961 cagcagatgc agtcgaggtc ccggctcctg ctgccgtgct tggtggtccc gagcctttga
     1021 tgcaagctac cgcttggctg aatgcatatt tccatcagcc agaggcgatc gaggagttcc
     1081 ccgttcccgc gctccatcat ccggttttcc aacaggaatc cttcacccgc caggtcctgt
     1141 g
//
