LOCUS       sequence-90151-e         968 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI A-B pNOS   c1
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(71..254)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(107..286)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(410..426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(410..426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(423..445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    434..450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(458..464,465..482,483..488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    503..524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(641..658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(892..911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggt aggtctctca
       61 gaagatccgg tgcagattat ttggattgag agtgaatatg agactctaat tggataccga
      121 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
      181 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
      241 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
      301 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
      361 ctcatgatcc cgctgagacc gtcctgcatg caagcttggc gtaatcatgg tcatagctgt
      421 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      481 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      541 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      601 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      661 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      721 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      841 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      901 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      961 gatacctg
//
