LOCUS       sequence-90177-e         821 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: Xpre-K
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    60..84
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(207..795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(287..306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgtactggg gtggattacg tgagacgata cgtctcatct ggtttaaact atcagtgttt
       61 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
      121 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccaa ccacaggaac
      181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg g
//
