LOCUS       sequence-90286-e         854 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for disruption of genes by CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pSAG1::CAS9-U6::sgUPRT
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..74)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(81..101)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(114..140)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(120..146)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcgcccttgc tcacggatcc gctgctgccg ttgctcccac taccaatgcc gtccactttc
       61 cgctttttct taggatcttc caccttgcgt tttttcttgg gggagccgct gcccaggctg
      121 gcataatcgg gcacgtcata gggataggcg tcgcctccca gctgagacag gtcgatccgt
      181 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg
      241 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc
      301 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat aggcttgtct
      361 ctgtgcttgt tgtaggcgct cagcaccttg tccagattag cgtcggccag gatcactctc
      421 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgtttgtg ctgttccaca
      481 aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata gtggctggcc
      541 aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt ctgcagttcg
      601 ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga gtacttaggc
      661 agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag aaagtcgatg
      721 ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc tttcacactc
      781 ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga ataggccacg
      841 gtggggctgt cgaa
//
