LOCUS       sequence-90310-e         859 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plasmid vector carrying N-Bio-1xproteinA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pCAndy-NBioTAP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    343..364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(379..384,385..402,403..409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     422..444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     441..457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     441..457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     476..496
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        478..496
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             565..738
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      301 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      361 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      421 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
      481 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gcccatggca
      541 ggccttgcgc aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg
      601 ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa
      661 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
      721 gatgctcagg cgccgaaagt agacgcgaat tgtgatatac ctacaactgc ttctctcgag
      781 agcggctcct cggctggaaa ggccggtgaa ggtgaaatcc ctgcccctct tgctggtacc
      841 gtttctaaga tactggtaa
//
