LOCUS       sequence-90749-e         933 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Detection construct to label dockerin type I containing fusion 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash 
            MA, Gaub HE
  TITLE     From genes to protein mechanics on a chip.
  JOURNAL   Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099.
  PUBMED    25194847
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099."
COMMENT     Sequence Label: pET28a-StrepII-TagRFP-CohI
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..61
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgtgg tctcacccgc agttcgaaaa
       61 agtttctaaa ggtgaagaac tgatcaaaga aaacatgcac atgaaactgt acatggaagg
      121 tactgttaac aaccaccact tcaaatgcac ctctgaaggt gaaggtaaac cgtacgaagg
      181 tactcagacc atgcgtatca aagttgttga aggtggtccg ctgccgttcg ctttcgacat
      241 cctggctacc tctttcatgt acggttctcg taccttcatc aaccacaccc agggtatccc
      301 ggacttcttc aaacagtctt tcccggaagg tttcacctgg gaacgtgtta ccacctacga
      361 agacggtggt gttctgaccg ctacccagga cacctctctg caagacggtt gcctgatcta
      421 caacgttaaa atccgtggtg ttaacttccc gtctaacggt ccggttatgc agaaaaaaac
      481 cctgggttgg gaagctaaca ccgaaatgct gtacccggct gacggtggtc tggaaggtcg
      541 ttctgacatg gctctgaaac tggttggtgg tggtcacctg atctgcaact tcaaaaccac
      601 ctaccgttct aaaaaaccgg ctaaaaacct gaaaatgccg ggtgtttact acgttgacca
      661 ccgtctggaa cgtatcaaag aagctgacaa agaaacctac gttgaacagc acgaagttgc
      721 tgttgctcgt tactgcgacc tgccgtctaa actgggtcac aaactgaacg gcagtgtagt
      781 accatcaaca cagcctgtaa caacaccacc tgcaacaaca aaaccacctg caacaacaat
      841 accgccgtca gatgatccga atgcaggatc cgacggtgtg gtagtagaaa ttggcaaagt
      901 tacgggatct gttggaacta cagttgaaat acc
//
