LOCUS       sequence-91051-e         860 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-C1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..356
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     misc_feature    357..422
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    546..667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(583..602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     637..656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(811..832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggagaaaagt ttcgtgataa
       61 ctgatccacg gctgccagac aatcccatca tcttcgcatc cgatggcttc ctggagctga
      121 ccgagtattc cagagaggag atcctgggcc gcaatggccg ctttctgcag ggaccagaga
      181 cagaccaggc cacagtgcag aagattcgcg atgccattag agatcagcgc gagattaccg
      241 tgcagctgat aaactacaca aaaagcggga agaaattctg gaacctcctg cacctccagc
      301 ccatgaggga ccagaagggt gagctccagt atttcatcgg agtgcagctg gatggatccg
      361 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      421 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
      481 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      541 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      661 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      721 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      781 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      841 aacgtggact ccaacgtcaa
//
