LOCUS       sequence-91401-e        8201 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of preCOX4-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Vowinckel J, Hartl J, Butler R, Ralser M
  TITLE     MitoLoc: A method for the simultaneous quantification of 
            mitochondrial network morphology and membrane potential in single 
            cells.
  JOURNAL   Mitochondrion. 2015 Sep;24:77-86. doi: 10.1016/j.mito.2015.07.001. 
            Epub 2015 Jul 13.
  PUBMED    26184437
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion."; date: "2015-09"; volume: "24:77-86. doi"; pages: "
            10.1016/j.mito.2015.07.001. Epub 2015 Jul 13"
COMMENT     Sequence Label: pMitoLOC
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..38)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(18..36)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(70..92)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    81..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    150..171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(288..305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(459..1047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(539..558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1218..2009,2010..2078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1841..1860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2079..2183)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2220..2723
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2764..2782
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2820..2842)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2942..2961
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     gene            3080..4199
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        3080..3423
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3424..3996
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      4002..4199
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      complement(4367..4822)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4504..4525)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4716..4735
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4948..4970
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4962..4979
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4963..4979
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4989..5008
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4989..5007
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5026..5273)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     5239..5257
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(5283..5999)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     6002..6018
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6247..6263)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6247..6263)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(6264..6613)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             7391..7456
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochfome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
     regulatory      7466..7475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7472..8182
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7617..7635)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7885..7904
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtccttttca
     2221 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
     2281 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
     2341 ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt
     2401 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
     2461 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
     2521 atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc
     2581 ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata
     2641 aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc
     2701 gtgtaagtta caggcaagcg atccgtctaa gaaaccatta ttatcatgac attaacctat
     2761 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     2821 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2881 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     2941 gcggcatcag agcagattgt actgagagtg caccatacca ccttttcaat tcatcatttt
     3001 ttttttattc ttttttttga tttcggtttc cttgaaattt ttttagcttg ccttgtcccc
     3061 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
     3121 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
     3181 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
     3241 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
     3301 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
     3361 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
     3421 accatgggta ccactcttga cgacacggct taccggtacc gcaccagtgt cccgggggac
     3481 gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg acaccgtctt ccgcgtcacc
     3541 gccaccgggg acggcttcac cctgcgggag gtgccggtgg acccgcccct gaccaaggtg
     3601 ttccccgacg acgaatcgga cgacgaatcg gacgacgggg aggacggcga cccggactcc
     3661 cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg gcttcgtggt cgtctcgtac
     3721 tccggctgga accgccggct gaccgtcgag gacatcgagg tcgccccgga gcaccggggg
     3781 cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt tcgcccgcga gcggggcgcc
     3841 gggcacctct ggctggaggt caccaacgtc aacgcaccgg cgatccacgc gtaccggcgg
     3901 atggggttca ccctctgcgg cctggacacc gccctgtacg acggcaccgc ctcggacggc
     3961 gagcaggcgc tctacatgag catgccctgc ccctaatcag tactgacaat aaaaagattc
     4021 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     4081 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     4141 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     4201 tgtcgattcg atactaacgc cgccatccag tgtcgaaaaa ctgtattata agtaaatgca
     4261 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt accctatgcg
     4321 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     4381 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4441 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4501 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4561 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4621 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4681 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4741 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4801 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     4861 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4921 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     4981 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt
     5041 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
     5101 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac
     5161 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag
     5221 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgacc
     5281 agttatttgt acaattcatc cataccatgg gtaataccag cagcagtaac aaattctaac
     5341 aagaccatgt ggtctctctt ttcgtttgga tctttggata aggcagattg agtggataag
     5401 taatggttgt ctggtaacaa gactggacca tcaccaattg gagtattttg ttgataatgg
     5461 tcagctaatt gaacagaacc atcttcaatg ttgtgtctaa ttttgaagtt aactttgata
     5521 ccattctttt gtttgtcagc catgatgtaa acattgtgag agttatagtt gtattccaat
     5581 ttgtgaccta aaatgttacc atcttcttta aaatcaatac cttttaattc gattctatta
     5641 actaaggtat caccttcaaa cttgacttca gctctggtct tgtagttacc gtcatctttg
     5701 aaaaaaatag ttctttcttg aacataacct tctggcatgg cagacttgaa aaagtcatgt
     5761 tgtttcatat gatctgggta tctcgcaaaa cattgaacac cataaccgaa agtagtgact
     5821 aaggttggcc atggaactgg caatttacca gtagtacaaa taaattttaa ggtcaattta
     5881 ccgtaagtag catcaccttc accttcaccg gagacagaaa atttgtgacc attaacatca
     5941 ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc tttagacatg
     6001 tcgaggtcga cggtatcgat aagcttgata tcgaattcgg aagagtaggc gcgcttctgg
     6061 aaagcctggc gggtggtggc gttgacgatg gaggtcatct gggtgcgctt gagggtctgg
     6121 aggggggagc cagtctggat ggtgcgcttg ctgacctgag caacgcggac agcagggcgg
     6181 gcaaccttgg cggaagcagc catccgggag gccaggcgag aggcgaggac acgagtggag
     6241 gccatggatc cactagttct agagtatgga tgggggtaat agaattgtat ctatgtatct
     6301 gacgaccctg tattacacga aggaaatagt agatgaaaag acaagagagc aagaaaaagg
     6361 aaaaacttcc tttctaacag acgctttact taaccttata tatatcttat ttttttcatc
     6421 gagcgtgttc aattggacaa ggtgccacct tttcgacact tcggttatta tgttacacag
     6481 ttttcatgag gatggcgcct tgactaactt aattagtcat ttgccaacca ccacagttcc
     6541 ccaatatcga cagcttcacg tgccatttgc cattttgcga tcatgtgacc tcaagagaaa
     6601 aagcaaaaaa taatgagagc taagggattc gaagtcgacg gtatcgataa gcttgatatc
     6661 cttttgttgt ttccgggtgt acaatatgga cttcctcttt tctggcaacc aaacccatac
     6721 atcgggattc ctataatacc ttcgttggtc tccctaacat gtaggtggcg gaggggagat
     6781 atacaataga acagatacca gacaagacat aatgggctaa acaagactac accaattaca
     6841 ctgcctcatt gatggtggta cataacgaac taatactgta gccctagact tgatagccat
     6901 catcatatcg aagtttcact accctttttc catttgccat ctattgaagt aataataggc
     6961 gcatgcaact tcttttcttt ttttttcttt tctctctccc ccgttgttgt ctcaccatat
     7021 ccgcaatgac aaaaaaatga tggaagacac taaaggaaaa aattaacgac aaagacagca
     7081 ccaacagatg tcgttgttcc agagctgatg aggggtatct cgaagcacac gaaacttttt
     7141 ccttccttca ttcacgcaca ctactctcta atgagcaacg gtatacggcc ttccttccag
     7201 ttacttgaat ttgaaataaa aaaaagtttg ctgtcttgct atcaagtata aatagacctg
     7261 caattattaa tcttttgttt cctcgtcatt gttctcgttc cctttcttcc ttgtttcttt
     7321 ttctgcacaa tatttcaagc tataccaagc atacaatcaa ggaattccat acaaatagat
     7381 aacaagcaca atgctttcac tacgtcaatc tataagattt ttcaagccag ccacaagaac
     7441 tttgtgtagc tctagaccac tagtcgccac catggtgagc aagggcgagg aggataacat
     7501 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tggacggcca
     7561 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     7621 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     7681 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     7741 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     7801 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     7861 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggaggc
     7921 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     7981 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     8041 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     8101 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     8161 catggacgag ctgtacaagt aagcggccgc caccgcggtg g
//
