LOCUS       sequence-91616-e        8126 bp    DNA     circular SYN 15-APR-2025
DEFINITION  FAK fret control sensor without kinase domain with 10 nm linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK-FERM10
FEATURES             Location/Qualifiers
     source          1..8126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             920..943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(962..964,965..1675)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2996..3016
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3272..3274,3275..3985)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4078..4095)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4084..4308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4441..4460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4592..4639
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4647..5669
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4647..5669
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5799..5920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5836..5855)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5890..5909
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5969..5985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5969..5985)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5982..6004)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5993..6009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6017..6023,6024..6041,6042..6047))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6062..6083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6200..6217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6371..6959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6451..6470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7130..7921,7922..7990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7753..7772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7991..8095)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg actacaagga cgatgacgac aagggaagcg gaggaagcgg
      961 agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1021 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1081 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1141 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     1201 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1261 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1321 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1381 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1441 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1501 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1561 cctgagctac cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1621 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtctag
     1681 aggaagcgga ggaagcggag gaagcggagg aagcggagga agcggaatgg cagcagctta
     1741 ccttgatcca aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg
     1801 gatggagcgt tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa
     1861 cagcagcgag ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg
     1921 aggcataata cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt
     1981 gcgactcagt catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc
     2041 caatgtgaga gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag
     2101 aattcggtac ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa
     2161 ttttttctat cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca
     2221 ggaaattgct ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg
     2281 caatgcatta gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg
     2341 attttttccg aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca
     2401 acagacattt cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga
     2461 gatcctctct ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag
     2521 ctggattatt tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga
     2581 caagggtgca aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc
     2641 aaacagtgaa gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga
     2701 gcctctgaca gtgacagcac catccttaac cattgcagag aatatggctg acttgataga
     2761 cggatactgc cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga
     2821 aggtgaaaga gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag
     2881 gtcgcacaca gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga
     2941 tacttataca atgggaagcg gaggaagcgg agaattcgga agcggaggaa gcggagaaaa
     3001 cctgtatttt cagggcggaa gcggagaaga ggaagagaaa aaaaaacagc aggaagagga
     3061 agcagaaagg ctgaggcgta ttcaagaaga aatggaaaag gaaagaaaaa gacgtgaaga
     3121 agatgaacaa cgtcgaagaa aggaggagga ggaaaggcgg atgaaacttg agatggaagc
     3181 aaagagaaaa caagaagaag aagagagaaa gaaaagggaa gatgatgaaa aacgcaagaa
     3241 gaagggctct ggcggctctg gcggcgcgcc cgtgagcaag ggcgaggagc tgttcaccgg
     3301 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3361 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3421 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
     3481 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3541 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3601 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3661 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca gcgacaacgt
     3721 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     3781 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3841 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     3901 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3961 tctcggcatg gacgagctgt acaagttaat taacggaagc ggaggaagcg gaggaagcgg
     4021 aggaagcgga tgagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     4081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4501 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4561 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     4621 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     4681 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     4741 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     4801 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     4861 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     4921 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     4981 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     5041 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     5101 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     5161 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     5221 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     5281 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     5341 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     5401 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     5461 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     5521 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     5581 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     5641 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
     5701 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5761 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5821 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5881 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5941 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6001 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6061 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6121 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6181 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6241 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6301 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6361 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6421 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6481 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6541 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6601 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6661 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6721 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6781 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6841 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6901 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6961 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7021 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7081 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7141 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7201 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7261 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7321 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7381 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7441 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7501 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7561 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7621 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7681 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7741 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7801 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7861 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7921 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7981 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8041 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8101 ttccccgaaa agtgccacct gacgtc
//
