LOCUS       sequence-91740-e         952 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  GUS-based microRNA target competitor used for testing function as 
            target mimicry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Liu Q, Wang F, Axtell MJ
  TITLE     Analysis of complementarity requirements for plant MicroRNA 
            targeting using a Nicotiana benthamiana quantitative transient 
            assay.
  JOURNAL   Plant Cell. 2014 Feb;26(2):741-53. doi: 10.1105/tpc.113.120972. Epub
            2014 Feb 7.
  PUBMED    24510721
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.113.120972"; journalName: "Plant Cell"; date: 
            "2014-02"; volume: "26"; issue: "2"; pages: "741-53"
COMMENT     Sequence Label: pGreen_GUS_competitor
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..637
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      657..909
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
       61 ctcaactcga tcgaggggat ctaccatgag cccagaacga cgcccggccg acatccgccg
      121 tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag
      181 cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg
      241 tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc
      301 ctacgcgggt ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta
      361 cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa
      421 gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc
      481 gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg
      541 cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt
      601 gccgccccgt ccggtcctgc ccgtcaccga aatctgatga cccctagagt caagcagatc
      661 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
      721 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
      781 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
      841 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
      901 tactagatcg accggcatgc aagctgataa cgttacacca caatatatcc tg
//
