LOCUS       sequence-91805-e        1037 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Induces Citrine expression from a promoter containing 2 lexA boxes 
            in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(94..343)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     309..327
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cggccgaagc tgggtaccaa gcagacgcta ctaaggaaaa caacaacgga agcgatgatg
       61 agagcgacga tgagagtgta aactgcgaac cttgcaaatt aaagccttcg agcgtcccaa
      121 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtt tgtacagaaa
      181 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta ttaaaaaaaa
      241 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg
      301 ggaggagggc gtgaatgtaa gcgtgacata actaattaca tgatatagac aaaggaaaag
      361 gggcctgtct cgagttagga tcctttgtac aattcatcca taccatgggt aataccagca
      421 gcagtaacaa attctaacaa gaccatgtgg tctctctttt cgtttggatc tttggataac
      481 ttagattgat aggataagta atggttgtct ggtaacaaga ctggaccatc accaattgga
      541 gtattttgtt gataatggtc agctaattga acagaaccat cttcaatgtt gtgtctaatt
      601 ttgaagttaa ctttgatacc attcttttgt ttgtcagcca tgatgtaaac attgtgagag
      661 ttatagttgt attccaattt gtgacctaaa atgttaccat cttctttaaa atcaatacct
      721 tttaattcga ttctattaac taaggtatca ccttcaaact tgacttcagc tctggtcttg
      781 tagttaccgt catctttgaa aaaaatagtt ctttcttgaa cataaccttc tggcatggca
      841 gacttgaaaa agtcatgttg tttcatatga tctgggtatc tagcaaaaca catcaaacca
      901 taacctaaag tagtgactaa ggttggccat ggaactggca atttaccagt agtacaaata
      961 aattttaagg tcaatttacc gtaagtagca tcaccttcac cttcaccgga gacagaaaat
     1021 ttgtgaccat taacatc
//
