LOCUS       sequence-91847-e         870 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expresses dominant negative human PKCi tagged with V5 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Wald FA, Oriolo AS, Mashukova A, Fregien NL, Langshaw AH, Salas PJ
  TITLE     Atypical protein kinase C (iota) activates ezrin in the apical 
            domain of intestinal epithelial cells.
  JOURNAL   J Cell Sci. 2008 Mar 1;121(Pt 5):644-54. doi: 10.1242/jcs.016246. 
            Epub 2008 Feb 12.
  PUBMED    18270268
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2008-03-1"; volume: "121(Pt 5):644-54. doi"; pages: " 
            10.1242/jcs.016246. Epub 2008 Feb 12"
COMMENT     Sequence Label: pcDNATM3.1/V5-His TOPO-PKCi-K274W
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag actcgagcgg ccgccactgt gctggatatc
      121 tgcagaattg tcttgaccct tgacacattc ttctgcagac atcaaaagag gattgatata
      181 ctcaaaacct tcaaattcag actgatcaat cttcctcaca atgtcatcgt catctggagt
      241 gagctggaca ggttcattag taaactgaga atcaaagttg tccaaaccaa attccccaga
      301 aatatttggt ttaaagggag gtaccacctg tttttgctcc atcatatccc aatcaacatt
      361 tcggaagaac gggtgtccct gaatatcagc aaatcctgtt tgaggatgac aacccaatcg
      421 ttccttaggg tccttattaa gaaaactctt cagaacactt gcagctttta cagacagaga
      481 acgtggtatg cgaatttgtt tttccaaaat aacttggaag agataatcct ctgtgttctg
      541 gtcagggtta tcggagctcc caacaatatc aaatggagac cttcctgcca tcatctcaaa
      601 catgagcact ccaagagccc accagtcaac actgaaacca taatcttctc ctcttaaaat
      661 ttcaggagca atgtaattag gagtaccaca gaaagtgctg gttgtatctc ctggccgtaa
      721 tccttcctta cacatgccgt agtcagtgag tttaatgtgg ccttcagagt ccagtaatac
      781 attgtccagt ttcaaatctc tataaattat ccctcgctca tgaagataat ttaatgctag
      841 actgatttct gcagagtaaa atctggcatg
//
