LOCUS       sequence-91889-e         605 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Flag-CHES1L mutant lacking the forkhead binding site and C-terminal 
            domain, with remaining NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, 
            Gaumont-Leclerc MF, Moro A, Ferbeyre G
  TITLE     CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and 
            protein biosynthesis.
  JOURNAL   Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. 
            Epub 2014 Jan 8.
  PUBMED    24403608
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-02-0110"; journalName: "Mol Biol Cell"; date: 
            "2014-03"; volume: "25"; issue: "5"; pages: "554-65"
COMMENT     Sequence Label: pLPC-Flag-CHES1LdFC-NLS
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             134..157
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             488..508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattcgcc atggactaca aagacgatga cgacaagggc atgggtccag tcatgcctcc
      181 cagtaagaag ccagaaagct caggaattag tgtctccagt ggactgagtc agtgttacgg
      241 gggcagcggt ttctccaagg cccttcagga agacgatgac ctcgactttt ctctgcctga
      301 catccgatta gaagaggggg ccatggaaga tgaagagctg accaacctga actggctgca
      361 cgagagcaag aacttgctga agagctttgg ggagtcggtc ctcaggagtg tcagccccgt
      421 ccaggacctg gacgatgaca cccccccatc ccctgcccac tctgacatgc cctacgatgc
      481 caggcagcca aagaagaagc gtaaggtata actcgagcat gcatctagag ggccgataaa
      541 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
      601 tggca
//
