LOCUS       sequence-92170-e         931 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Tanaka T, Serneo FF, Higgins C, Gambello MJ, Wynshaw-Boris A, 
            Gleeson JG
  TITLE     Lis1 and doublecortin function with dynein to mediate coupling of 
            the nucleus to the centrosome in neuronal migration.
  JOURNAL   J Cell Biol. 2004 Jun 7. 165(5):709-21.
  PUBMED    15173193
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Jun 7. 165(5):709-21."
COMMENT     Sequence Label: EGFP-DCX
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..770)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(54..56,57..59,60..770)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(360..379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     707..728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             798..815
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             825..842
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      121 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      241 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      361 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      481 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      541 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      661 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      721 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
      781 gatccatggg cagcagccat catcatcatc atcacagcag cggcctggtg ccgcgcggca
      841 gccatatgga acttgatttt ggacactttg acgaaagaga taagacatcc aggaacatgc
      901 gaggctcccg gatgaatggg ttgcctagcc c
//
