LOCUS       sequence-92248-e         856 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Actin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mApple-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..836)
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(404..423)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      833..842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
      181 tactgttcca cgatggtgta gtcctcgttg tgggacacga tgtccaactt gatgtcgacg
      241 atgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcggcg
      301 gcgtagtggc cgccgtcctt cagcttcagc ctcttcttga tctcgctctt cagggcgccg
      361 tcctcggggt acatccgctc ctcggaggcc tcccagccca tggtcttctt ctgcattacg
      421 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa cacgccgtcc
      481 tgcagggagg agtcctggtt aacgtgaata atgccgccgt cctcgaagtt catcacgcgc
      541 tcccacctga agccctcggg gaaggacagc ttgaagtagt cggggatgtc ggctgggtgc
      601 ttaatgtaga ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
      661 agggggccac ccttggtcac cttcagctta gcggtctgaa aggcctcgta ggggcggccc
      721 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
      781 aagcgcatga actccttgat gatggccatg ttattctcct cgcccttgct caccatggtg
      841 gcgaccggtg gatccc
//
