LOCUS       sequence-9244-ex        4676 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC95
FEATURES             Location/Qualifiers
     source          1..4676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             508..1779
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        2421..2525
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2526..2594,2595..3386)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2744..2763)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3557..4145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4046..4065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4299..4316
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4433..4454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4469..4474,4475..4492,4493..4499)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4507..4523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4512..4534
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4531..4547
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4531..4547
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcaccaa aaacggaacg ttgagctgga
       61 cggaaatagt ggtaaagtga catgattata gtttgaagat ttctaatttc acaattagag
      121 caaatgttgt tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga
      181 acattcgagc tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt
      241 gtacacaata aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag
      301 tattttcaaa cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg
      361 gtatataagg aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa
      421 acttcgaaaa tcctcatcgg gatcctatcg attcgcggcc gctgtacacc cgggtgccct
      481 atagtgagtc gtattggtac caaaaacatg ccacaatttg atatattatg taaaacacca
      541 cctaaggtgc ttgttcgtca gtttgtggaa aggtttgaaa gaccttcagg tgagaaaata
      601 gcattatgtg ctgctgaact aacctattta tgttggatga ttacacataa cggaacagca
      661 atcaagagag ccacattcat gagctataat actatcataa gcaattcgct gagtttcgat
      721 attgtcaata aatcactcca gtttaaatac aagacgcaaa aagcaacaat tctggaagcc
      781 tcattaaaga aattgattcc tgcttgggaa tttacaatta ttccttacta tggacaaaaa
      841 catcaatctg atatcactga tattgtaagt agtttgcaat tacagttcga atcatcggaa
      901 gaagcagata agggaaatag ccacagtaaa aaaatgctta aagcacttct aagtgagggt
      961 gaaagcatct gggagatcac tgagaaaata ctaaattcgt ttgagtatac ttcgagattt
     1021 acaaaaacaa aaactttata ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga
     1081 ttcagcgata ttaagaacgt tgatccgaaa tcatttaaat tagtccaaaa taagtatctg
     1141 ggagtaataa tccagtgttt agtgacagag acaaagacaa gcgttagtag gcacatatac
     1201 ttctttagcg caaggggtag gatcgatcca cttgtatatt tggatgaatt tttgaggaat
     1261 tctgaaccag tcctaaaacg agtaaatagg accggcaatt cttcaagcaa taaacaggaa
     1321 taccaattat taaaagataa cttagtcaga tcgtacaata aagctttgaa gaaaaatgcg
     1381 ccttattcaa tctttgctat aaaaaatggc ccaaaatctc acattggaag acatttgatg
     1441 acctcatttc tttcaatgaa gggcctaacg gagttgacta atgttgtggg aaattggagc
     1501 gataagcgtg cttctgccgt ggccaggaca acgtatactc atcagataac agcaatacct
     1561 gatcactact tcgcactagt ttctcggtac tatgcatatg atccaatatc aaaggaaatg
     1621 atagcattga aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag
     1681 ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta
     1741 ctagactacc tttcatccta cataaataga cgcatataag tacgcattta agcataaaca
     1801 cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg
     1861 acgtgaacag tgagctgtat gtgcggggat ccactagttc tagccggcca tacaagtaat
     1921 ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     1981 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     2041 caatccaaat ctaaacatga gcggtttttt ttctctttcc gtctcccaat tcgtattccg
     2101 ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg
     2161 acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg
     2221 acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat
     2281 aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg
     2341 atgagaattg tcctcgcttg ggcccaaaag gccttttttt tttggcgccg acgtcaggtg
     2401 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2461 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2521 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2581 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2641 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2701 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2761 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2821 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2881 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2941 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3001 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3061 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3121 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3181 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3241 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3301 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3361 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3421 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3481 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3541 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3601 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3661 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3721 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3781 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3841 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3901 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     3961 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4021 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4081 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4141 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4201 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4261 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4321 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4381 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4441 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4501 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4561 agctgtaagt ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt
     4621 aaaaatggct gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
