LOCUS       sequence-9257-ex       10766 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC240
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1862..1886)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1896..1943)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2019..2039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2061..2081
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2103..2123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2145..2165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2208..2236)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2984..3004
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(3901..3948)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4024..4044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4066..4086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4108..4128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4150..4170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4192..4869
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             4912..5586
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     CDS             complement(6471..6482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8517..8621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8622..8690,8691..9482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8840..8859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9653..10241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10142..10161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10395..10412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10529..10550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10565..10570,10571..10588,10589..10595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10603..10619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10608..10630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10627..10643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10627..10643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcggatc cactagttct
       61 agcttcaaaa gcatcaacaa gtttgtacaa aaaagcaggc tacttgtgcc ttgattctcc
      121 tgttgaacgt gagtgttttt tttttaattt gaaaatgtta agcgtgggcc aaaatatttc
      181 aaagaattta catccggaaa aaattttttt gtaaattttc tgaaaatcac agcttaaaaa
      241 attcaaaaca aaagttaagt tagattcata aaatttccat ttgaaaagac atctaacaga
      301 tctaagaata caacaccttc tctcaaactc cacttaatct tgaaaaataa atcttaaaat
      361 gtcaaaatcc ctgcaataac aaaatcgaaa gaaggagagg gagaaaatct ggaaaaaaag
      421 attattcgca cttctaagcc ttaaatatcc gtctattttc ccctgaattt tgcaactttt
      481 ttatctgaag ctctggtgct cttccgattc ttttcccata ctacttatag ggatgagcca
      541 atgaataaat gagcaaagtt ccgaaaggtg tctgaactta atgggctcac ttccaaaaaa
      601 ttttgaagtc atataacatt actaaatttc ttggtagaaa aaacccattt gagctacaca
      661 tttttccatg acaccttgct tattcaccag tttggcaaac aaaagaatcg gggggtctca
      721 cacaaaaata tattttgttg attaaacgat atgatggggg tggggaacaa atgggaggct
      781 ccatcaaaaa gctcttagtt caatcaagtt ctgaaagttt aaaagaaaac aaaaatcgct
      841 atttctttca ttccatttgt ccccctcctc ttttactttt ttcttttcca ccttcttctt
      901 gttgtcttaa aaacgcataa cttcatctgg tccttcgatg aagatctaag ctataatcgc
      961 cttatgcaca tcacatgtac atatccggtt tgaagatttc aacaacccaa ccagattttc
     1021 tcgattttct tggcctcgaa aacttaacct agaaagtttt caaataaata tatgatatta
     1081 ataaaaatta acttttcaca gttttgtatt ccgaaagttt ttaaaaccta attttcttca
     1141 aaaaaagttt ttctctaggt caattttcaa ttctaatttc aaatcgcaac actctcctcc
     1201 caaataagta gttatttgcc gccgctccga ttatgattac tgtagtggtg gggtgtttat
     1261 aattgcttat attaagtaaa ctttccttct ttttttggga ttgttacttc ttgtttgtca
     1321 acggccagtt tacttcctaa aataagatgt caaatgttga acaaggggat tatcaatagt
     1381 ttgaaatgac tacctttagt tgttatagtt ttaaaattag aacattttaa tatgttattt
     1441 tttaaacagt aggaagctgt cgactttaaa ttcttccgaa taaaaacgga gctcaataaa
     1501 attaatgacg tggcaaaaca gtgctgacag aagagggcaa aaataaaata aaaaacgttt
     1561 tggcagctac taacatcccc aaacacccca caaatgagct attttatgag ttttgttagg
     1621 ctctccctgc ctttcttcac ttttttcttg aagccatgaa ccccttcatg acacctaact
     1681 gggtatcatt atccatttgt gcgcgtggcc ctcccctctt tctattactt ttccctaaat
     1741 aaagcttgag ctcccctcct ctcacaaacc ctgtaatctt caaatcaaag tcgacgggta
     1801 atttgggtta gagtaaagtt aatcgagctt tcatttttca gaacgtcagc accaaccacc
     1861 gacccagctt tcttgtacaa agtggttgat gctctgaagt tcctatactt tctagagaat
     1921 aggaacttcg gaataggaac ttcaagatcc ccgggtatgt ttcgaatgat actaacataa
     1981 catagaacat tttcaggagc tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac
     2041 cggcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggcagaa aaaatgactg
     2101 ctccaaagaa gaagcgtaag gtaccggcag aaaaaatgac tgctccaaag aagaagcgta
     2161 aggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     2221 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     2281 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     2341 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
     2401 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
     2461 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     2521 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
     2581 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
     2641 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     2701 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     2761 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
     2821 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     2881 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     2941 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     3001 taacagctgc tgggattaca catggcatgg acgaactata caaaagccca caagctttgg
     3061 aattcgctag ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc
     3121 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     3181 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatgagcg gttttttttc
     3241 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     3301 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     3361 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     3421 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     3481 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc cctttcgtct
     3541 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3601 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3661 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     3721 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     3781 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3841 agacgactct agaccgcggt tttggcgcgg atccactagt tctagcttca aaagcgctct
     3901 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa gatccccggg
     3961 tatgtttcga atgatactaa cataacatag aacattttca ggagctcaga aaaaatgact
     4021 gctccaaaga agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt
     4081 aaggtaccgg cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa
     4141 atgactgctc caaagaagaa gcgtaaggta ccggtagacg ataaggatcc gatggtggcc
     4201 tcctccgagg acgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     4261 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4321 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     4381 ccccagttcc agtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgactac
     4441 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4501 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     4561 aagttccgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     4621 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     4681 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     4741 gccaagaagc ccgtgcagct gcccggctac tactacgtgg acaccaagct ggacatcacc
     4801 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     4861 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     4921 gaggacgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     4981 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     5041 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     5101 ttccagtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ctacaagaag
     5161 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     5221 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagttc
     5281 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     5341 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     5401 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     5461 aagcccgtgc agctgcccgg ctactactac gtggacacca agctggacat cacctcccac
     5521 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     5581 ctgtaggaat tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg
     5641 aattttcaaa ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt
     5701 tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac atgagcggtt
     5761 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
     5821 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
     5881 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
     5941 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa
     6001 ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccct
     6061 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6181 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6241 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6301 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6361 gtttcttaga caacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     6421 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     6481 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     6541 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     6601 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     6661 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     6721 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     6781 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     6841 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     6901 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     6961 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca acaggtttct
     7021 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     7081 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     7141 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     7201 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     7261 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     7321 agcaacaaca atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     7381 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     7441 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     7501 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     7561 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     7621 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     7681 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     7741 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     7801 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     7861 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     7921 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     7981 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     8041 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     8101 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     8161 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     8221 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     8281 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     8341 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     8401 tttcaatttc aactgaaaga ttttttcatt agagaatgtc taggcatgct ctagagggcc
     8461 caaaaggcct tttttttttg gcgccgacgt caggtggcac ttttcgggga aatgtgcgcg
     8521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9781 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10021 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
    10081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10321 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10381 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10441 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10501 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10561 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10621 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
    10681 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
    10741 cgatggatac gctaacaact tggaaa
//
