LOCUS       sequence-92574-e         982 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses LexA-ER-B112 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 17. pii: gku616."
COMMENT     Sequence Label: FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             618..869
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
ORIGIN
        1 cgcctatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaga agcgggtaag
       61 ctgccacagc aattaatgca caacatttaa cctacattct tccttatcgg atcctcaaaa
      121 cccttaaaaa catatgcctc accctaacat attttccaat taaccctcaa tatttctctg
      181 tcacccggcc tctattttcc attttcttct ttacccgcca cgcgtttttt tctttcaaat
      241 ttttttcttc cttcttcttt ttcttccacg tcctcttgca taaataaata aaccgttttg
      301 aaaccaaact cgcctctctc tctccttttt gaaatatttt tgggtttgtt tgatcctttc
      361 cttcccaatc tctcttgttt aatatatatt catttatatc acgctctctt tttatcttcc
      421 tttttttcct ctctcttgta ttcttccttc ccctttctac tcaaaccaag aagaaaaaga
      481 aaaggtcaat ctttgttaaa gaataggatc ttctactaca tcagctttta gatttttcac
      541 gcttactgct tttttcttcc caagatcgaa aatttactga attaacaact agtggatccc
      601 ccgggctgca ggaattcatg aaagcgttaa cggccaggca acaagaggtg tttgatctca
      661 tccgtgatca catcagccag acaggtatgc cgccgacgcg tgcggaaatc gcgcagcgtt
      721 tggggttccg ttccccaaac gcggctgaag aacatctgaa ggcgctggca cgcaaaggcg
      781 ttattgaaat tgtttccggc gcatcacgcg ggattcgtct gttgcaggaa gaggaagaag
      841 ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact tctggcgcaa cagcatattg
      901 aaggtcatta tcaggtcgat ccttccttat tcaagccgaa tgctgatttc ctgctgcgcg
      961 tcagcgggat gtcgatgaaa ga
//
