LOCUS       sequence-92663-e         745 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Cytoskeleton , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(35..55)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(128..331)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(157..177)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        332..635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttgctcac catggtggcg accggtccct ttggcttgct cttgctgctt cccatggtgg
       61 cgaattcgaa gcttgagctc gagatctgag tccggtagcg ctagcggatc tgacggttca
      121 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      181 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      241 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
      301 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
      361 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
      421 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
      481 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
      541 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      601 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
      661 ctaatgaccc cgtaattgat tactattaat aactaatgca tggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaa
//
