LOCUS       sequence-92866-e         766 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus 
            pluvialis fused to a Trc promoter. Produces canthaxanthin in E. 
            coli. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosynth 
            Res."; date: "2007-05"; volume: "92(2)"; pages: "245-59. Epub 2007 
            Jul 17"
COMMENT     Sequence Label: pAC-CANTHipi
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccga gctcaagcgt
      121 ttgtgcgcct cgacgtggcc agtctgcact gccttgaacc cgcgagtctc ccgccgcact
      181 gactgccata gcacagctag acgaatgcag ctagcagcga cagtaatgtt ggagcagctt
      241 accggaagcg ctgaggcact caaggagaag gagaaggagg ttgcaggcag ctctgacgtg
      301 ttgcgtacat gggcgaccca gtactcgctt ccgtcagaag agtcagacgc ggcccgcccg
      361 ggactgaaga atgcctacaa gccaccacct tccgacacaa agggcatcac aatggcgcta
      421 gctgtcatcg gctcctgggc cgcagtgttc ctccacgcca tttttcaaat caagcttccg
      481 acctccttgg accagctgca ctggctgccc gtgtcagatg ccacagctca gctggttagc
      541 ggcagcagca gcctgctgca catcgtcgta gtattctttg tcctggagtt cctgtacaca
      601 ggccttttta tcaccacgca tgatgctatg catggcacca tcgccatgag aaacaggcag
      661 cttaatgact tcttgggcag agtatgcatc tccttgtacg cctggtttga ttacaacatg
      721 ctgcaccgca agcattggga gcaccacaac cacactggcg aggtgg
//
