LOCUS       sequence-93010-e         846 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD 
            double mutant T207D, K227A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pSG5-Myr-FLAG-p110-alpha-DM
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..74
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctaaccaaa ggaccccagc cagcgccggc gcagaatccg aggttacctt gattacaagg
       61 atgacgacga taagcctcca cgaccatctt cgggtgaact gtggggcatc cacttgatgc
      121 ccccacgaat cctagtggaa tgtttactcc ccaatggaat gatagtgact ttagaatgcc
      181 tccgtgaggc cacactcgtc accatcaaac atgaactgtt cagagaggcc aggaaatacc
      241 ctctccatca gcttctgcaa gacgaaactt cttacatttt cgtaagtgtc acccaagaag
      301 cagaaaggga agaatttttt gatgaaacaa gacgactttg tgaccttcgg ctttttcaac
      361 cctttttaaa agttattgaa ccagtaggca accgtgaaga aaagatcctc aatcgagaaa
      421 ttggttttgt tattggcatg ccagtgtgtg aatttgatat ggttaaagat ccagaagtcc
      481 aagactttcg aaggaacatt ctgaatgttt gcaaagaagc tgtggacctg cgggatctca
      541 actcgcctca tagcagagca atgtatgtct accctccaaa tgtcgagtct tccccagaac
      601 tgccaaagca catctacaac aagttagata aaggacaaat catagtggtg atttgggtaa
      661 tagtctctcc aaacaacgac aagcagaagt acgatctgaa gatcaatcat gactgtgtgc
      721 cagagcaagt cattgctgaa gccatcaggg caaagactcg gagcatgttg ttgtcctctg
      781 agcagctgaa actctgtgtc ttagaatatc agggcaagta tattctgaaa gtgtgtggct
      841 gtgacg
//
