LOCUS       sequence-93098-e       10488 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Retroviral vector that expresses catalytically inactive form of 
            HA-tagged human USP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
  TITLE     Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation 
            of the INK4a tumour suppressor.
  JOURNAL   EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
            2010 Jul 2.
  PUBMED    20601937
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.129"; journalName: "EMBO J"; date: "2010-08-4- 
            4"; volume: "29"; issue: "15"; pages: "2553-65"
COMMENT     Sequence Label: pQHA-USP7 CS puroR
FEATURES             Location/Qualifiers
     source          1..10488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5616..6189,6190..6192,6193..6201)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5783..5800)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6010..6029
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6221..6820
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6221..6240)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6717..6737
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7194..7619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7722..7744)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7880..7900)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7885..8214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8065..8200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8236..8255
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8336..8353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8507..9095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8587..8606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9266..10057,10058..10126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9889..9908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10127..10231)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10299..10317
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10417..10488,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attgtacgac gtaccagatt
     2281 acgctaccgg tatgaaccac cagcagcagc agcagcagca gaaagcgggc gagcagcagt
     2341 tgagcgagcc cgaggacatg gagatggaag cgggagatac agatgaccca ccaagaatta
     2401 ctcagaaccc tgtgatcaat gggaatgtgg ccctgagtga tggacacaac accgcggagg
     2461 aggacatgga ggatgacacc agttggcgct ccgaggcaac ctttcagttc actgtggagc
     2521 gcttcagcag actgagtgag tcggtcctta gccctccgtg ttttgtgcga aatctgccat
     2581 ggaagattat ggtgatgcca cgcttttatc cagacagacc acaccaaaaa agcgtaggat
     2641 tctttctcca gtgcaatgct gaatctgatt ccacgtcatg gtcttgccat gcacaagcag
     2701 tgctgaagat aataaattac agagatgatg aaaagtcgtt cagtcgtcgt attagtcatt
     2761 tgttcttcca taaagaaaat gattggggat tttccaattt tatggcctgg agtgaagtga
     2821 ccgatcctga gaaaggattt atagatgatg acaaagttac ctttgaagtc tttgtacagg
     2881 cggatgctcc ccatggagtt gcgtgggatt caaagaagca cacaggctac gtcggcttaa
     2941 agaatcaggg agcgactagt tacatgaaca gcctgctaca gacgttattt ttcacgaatc
     3001 agctacgaaa ggctgtgtac atgatgccaa ccgaggggga tgattcgtct aaaagcgtcc
     3061 ctttagcatt acaaagagtg ttctatgaat tacagcatag tgataaacct gtaggaacaa
     3121 aaaagttaac aaagtcattt gggtgggaaa ctttagatag cttcatgcaa catgatgttc
     3181 aggagctttg tcgagtgttg ctcgataatg tggaaaataa gatgaaaggc acctgtgtag
     3241 agggcaccat acccaaatta ttccgcggca aaatggtgtc ctatatccag tgtaaagaag
     3301 tagactatcg gtctgataga agagaagatt attatgatat ccagctaagt atcaaaggaa
     3361 agaaaaatat atttgaatca tttgtggatt atgtggcagt agaacagctc gatggggaca
     3421 ataaatacga cgctggggaa catggcttac aggaagcaga gaaaggtgtg aaattcctaa
     3481 cattgccacc agtgttacat ctacaactga tgagatttat gtatgaccct cagacggacc
     3541 aaaatatcaa gatcaatgat aggtttgaat tcccagagca gttaccactt gatgaatttt
     3601 tgcaaaaaac agatcctaag gaccctgcaa attatattct tcatgcagtc ctggttcata
     3661 gtggagataa tcatggtgga cattatgtgg tttatctaaa ccccaaaggg gatggcaaat
     3721 ggtgtaaatt tgatgacgac gtggtgtcaa ggtgtactaa agaggaagca attgagcaca
     3781 attatggggg tcacgatgac gacctgtctg ttcgacactg cactaatgct tacatgttag
     3841 tctacatcag ggaatcaaaa ctgagtgaag ttttacaggc ggtcaccgac catgatattc
     3901 ctcagcagtt ggtggagcga ttacaagaag agaaaaggat cgaggctcag aagcggaagg
     3961 agcggcagga agcccatctc tatatgcaag tgcagatagt cgcagaggac cagttttgtg
     4021 gccaccaagg gaatgacatg tacgatgaag aaaaagtgaa atacactgtg ttcaaagtat
     4081 tgaagaactc ctcgcttgct gagtttgttc agagcctctc tcagaccatg ggatttccac
     4141 aagatcaaat tcgattgtgg cccatgcaag caaggagtaa tggaacaaaa cgaccagcaa
     4201 tgttagataa tgaagccgac ggcaataaaa caatgattga gctcagtgat aatgaaaacc
     4261 cttggacaat attcctggaa acagttgatc ccgagctggc tgctagtgga gcgaccttac
     4321 ccaagtttga taaagatcat gatgtaatgt tatttttgaa gatgtatgat cccaaaacgc
     4381 ggagcttgaa ttactgtggg catatctaca caccaatatc ctgtaaaata cgtgacttgc
     4441 tcccagttat gtgtgacaga gcaggattta ttcaagatac tagccttatc ctctatgagg
     4501 aagttaaacc gaatttaaca gagagaattc aggactatga cgtgtctctt gataaagccc
     4561 ttgatgaact aatggatggt gacatcatag tatttcagaa ggatgaccct gaaaatgata
     4621 acagtgaatt acccaccgca aaggagtatt tccgagatct ctaccaccgc gttgatgtca
     4681 ttttctgtga taaaacaatc cctaatgatc ctggatttgt ggttacgtta tcaaatagaa
     4741 tgaattattt tcaggttgca aagacagttg cacagaggct caacacagat ccaatgttgc
     4801 tgcagttttt caagtctcaa ggttataggg atggcccagg taatcctctt agacataatt
     4861 atgaaggtac tttaagagat cttctacagt tcttcaagcc tagacaacct aagaaacttt
     4921 actatcagca gcttaagatg aaaatcacag actttgagaa caggcgaagt tttaaatgta
     4981 tatggttaaa cagccaattt agggaagagg aaataacact atatccagac aagcatgggt
     5041 gtgtccggga cctgttagaa gaatgtaaaa aggccgtgga gcttggggag aaagcatcag
     5101 ggaaacttag gctgctagaa attgtaagct acaaaatcat tggtgttcat caagaagatg
     5161 aactattaga atgtttatct cctgcaacga gccggacgtt tcgaatagag gaaatccctt
     5221 tggaccaggt ggacatagac aaagagaatg agatgcttgt cacagtggcg catttccaca
     5281 aagaggtctt cggaacgttc ggaatcccgt ttttgctgag gatacaccag ggcgagcatt
     5341 ttcgagaagt gatgaagcga atccagagcc tgctggacat ccaggagaag gagtttgaga
     5401 agtttaaatt tgcaattgta atgacgggcc gacaccagta cataaatgaa gacgagtatg
     5461 aagtaaattt gaaagacttt gagccacagc ccggtaatat gtctcatcct cggccttggc
     5521 tagggctcga ccacttcaac aaagccccaa agaggagtcg ctacacttac cttgaaaagg
     5581 ccattaaaat ccataactga ggatccggaa ttccgcccct ctccctcccc cccccctaac
     5641 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5701 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5761 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5821 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5881 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5941 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6001 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6061 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6121 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     6181 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac
     6241 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     6301 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     6361 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6421 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6481 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6541 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6601 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6661 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6721 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga
     6781 ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     6841 gcgcccgacc gaaaggagcg cacgacccca tggctccgac cgaagccgac ccgggcggcc
     6901 ccgccgaccc cgcacccgcc cccgaggccc accgactcta gtcgagggct gcagcgctgc
     6961 agaggccgag tgcagaactg ctccaaaggg acctcaaggc tttccgaggg acactaggct
     7021 gactccatcg agccagtgta gagataagct tatcgattag tccaatttgt taaagacagg
     7081 atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa gcctatagag
     7141 tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     7201 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     7261 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagggtcg
     7321 accctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     7381 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     7441 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     7501 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     7561 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
     7621 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
     7681 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7741 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7801 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     7861 ggagtgtaga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     8221 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     8281 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     8341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8941 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     9601 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9661 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9721 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9781 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9841 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     9901 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9961 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10021 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10081 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10141 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10201 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10321 ttcgtcttca agaattagct tggccattgc atacgttgta tccatatcat aatatgtaca
    10381 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
    10441 aatagtaatc aattacgggg tcattagttc atagcccata tatggagt
//
