LOCUS       sequence-93223-e         725 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites
            removed and S807 restored .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF
  TITLE     Cyclin D activates the Rb tumor suppressor by mono-phosphorylation.
  JOURNAL   Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.
  PUBMED    24876129
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2014
            May 29:e02872. doi: 10.7554/eLife.02872."
COMMENT     Sequence Label: pCMV  HA hRB delta CDK + S807
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          130..226
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             290..316
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgga ctctagagga tccggtacta gaggaactga aaaaccagaa
      121 agttaactgg taagtttagt ctttttgtct tttatttcag gtcccggatc cggtggtggt
      181 gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac ttctaggcct gtacggaagt
      241 gttacttctg ctctaaaagc tgcggaattg tacccgcggg cccaccatgt acccatacga
      301 tgttccagat tacgctctta tggccatgga ggcccgaatt cggtcgaccc cgcccaaagc
      361 cccccgaaaa acggccgcca ccgccgccgc tgccgccgcg gaacccccgg caccgccgcc
      421 gccgccccct cctgaggagg acccagagca ggacagcggc ccggaggacc tgcctctcgt
      481 caggcttgag tttgaagaaa cagaagaacc tgattttact gcattatgtc agaaattaaa
      541 gataccagat catgtcagag agagagcttg gttaacttgg gagaaagttt catctgtgga
      601 tggagtattg ggaggttata ttcaaaagaa aaaggaactg tggggaatct gtatctttat
      661 tgcagcagtt gacctagatg agatgtcgtt cacttttact gagctacaga aaaacataga
      721 aatca
//
