LOCUS       sequence-93237-e         954 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses diphtheria toxin A subunit in Cre positive cells, and 
            mCherry in all the infected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Wu Z, Autry AE, Bergan JF, Watabe-Uchida M, Dulac CG
  TITLE     Galanin neurons in the medial preoptic area govern parental 
            behaviour.
  JOURNAL   Nature. 2014 May 15;509(7500):325-30. doi: 10.1038/nature13307.
  PUBMED    24828191
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-05-15- 15"; volume: "509"; issue: "7500"; 
            pages: "325-30"
COMMENT     Sequence Label: pAAV-mCherry-flex-dtA
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             206..778
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatctagcat
       61 gcgataacct cgtatagcat acattatacg aagtaattct ttgcaccatt ctaaagaata
      121 gtactgataa tttctgggtt aaggcaaatt acctcgtata ggatacttta tacgaagtta
      181 tgtcgacgtc ctcgccatgg atcctgatga tgttgttgat tcttctaaat cttttgtgat
      241 ggaaaacttt tcttcgtacc acgggactaa acctggttat gtagattcca ttcaaaaagg
      301 tatacaaaag ccaaaatctg gtacacaagg aaattatgac gatgattgga aagggtttta
      361 tagtaccgac aataaatacg acgctgcggg atactctgta gataatgaaa acccgctctc
      421 tggaaaagct ggaggcgtgg tcaaagtgac gtatccagga ctgacgaagg ttctcgcact
      481 aaaagtggat aatgccgaaa ctattaagaa agagttaggt ttaagtctca ctgaaccgtt
      541 gatggagcaa gtcggaacgg aagagtttat caaaaggttc ggtgatggtg cttcgcgtgt
      601 agtgctcagc cttcccttcg ctgaggggag ttctagcgtt gaatatatta ataactggga
      661 acaggcgaaa gcgttaagcg tagaacttga gattaatttt gaaacccgtg gaaaacgtgg
      721 ccaagatgcg atgtatgagt atatggctca agcctgtgca ggaaatcgtg tcaggcgatc
      781 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
      841 tttaaagctc taagatctat tacttcgtat aatgtatgct atacgaggtt attacttgta
      901 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttc
//
